Rabbit anti-Mouse, Rat ROCK1 Polyclonal Antibody | anti-ROCK1 antibody

ROCK1 Polyclonal Antibody

Cat. Num. AAA2556145

• Gene Names: Rock1; Rock-I; 1110055K06Rik
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: ROCK1; Polyclonal Antibody; ROCK1 Polyclonal Antibody; coiled-coil-containing protein kinase 1; coiled-coil-containing protein kinase I; MGC131603; MGC43611; p160 Rhoassociated coiled coil-forming protein kinase; p160 ROCK-1; p160 ROCK1; p160ROCK; PRO0435; Renal carcinoma antigen NY REN 35; Renal carcinoma antigen NY-REN-35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho kinase; Rho-alpha kinase; Rho-associated; Rho-associated protein kinase 1; ROCK I; ROCK-I; Rok; rokalpha; anti-ROCK1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 300 mug/mL (varies by lot)
• Sequence Length: 1,353

• Applicable Applications for anti-ROCK1 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:100-1:400
• Immunogen: KLH conjugated synthetic peptide corresponding to Mouse Rock1
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded mouse kidney using ROCK1 Polyclonal Antibody at dilution of 1:300.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded rat spleen using ROCK1 Polyclonal Antibody at dilution of 1:300.)

Related Product Information for anti-ROCK1 antibody

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.

Product Categories/Family for anti-ROCK1 antibody

Cancer; Cell Biology; Signal transduction

NCBI and Uniprot Product Information

• NCBI GI #: 6677759
• NCBI GeneID: 19877
• NCBI Accession #: NP_033097.1
• NCBI GenBank Nucleotide #: NM_009071.2
• UniProt Accession #: P70335; Q8C3G4; Q8C7H0
• Molecular Weight: 158,042 Da
• NCBI Official Full Name: rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated coiled-coil containing protein kinase 1
• NCBI Official Symbol: Rock1
• NCBI Official Synonym Symbols: Rock-I; 1110055K06Rik
• NCBI Protein Information: rho-associated protein kinase 1
• UniProt Protein Name: Rho-associated protein kinase 1
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: Rock1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK

Uniprot Description

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Promotes keratinocyte terminal differentiation (). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.