Mouse Carbonic Anhydrase VIII Monoclonal Antibody | anti-CA8 antibody

Carbonic Anhydrase VIII

Cat. Num. AAA4380717

• Gene Names: CA8; CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• Reactivity: Human. Others not tested.
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Carbonic Anhydrase VIII; Monoclonal Antibody; CA-VIII; CALS; CARP; MGC120502; MGC99509; anti-CA8 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: CPTC-CA8-2
• Specificity: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in CA8 (carbonic anhydrase 8) gene causes neuropathology, such as ataxia, mild mental retardation and the predisposition to quadrupedal gait. It is also associated with the development of colorectal and lung cancers. Additionally, it is upregulated in various cancers.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 290

• Applicable Applications for anti-CA8 antibody: Western Blot (WB), Immunohistology (Formalin-Fixed)
• Application Notes: WB: 0.5-1.0ug/ml; Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Immunogen: Recombinant human full-length CA8 protein
• Positive Control: Human cerebellum. Selective expression in purkinje Cells. HEK-293 and K-562 cell lines.
• Cellular Localization: Cytoplasm, membrane.
• Chromosome Location: 8q12.1
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Cerebllumstained with CA8 Mouse Monoclonal Antibody (CPTC-CA8-2).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Cerebllumstained with CA8 Mouse Monoclonal Antibody (CPTC-CA8-2).)

Testing Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Carbonic Anhydrase VIII Mouse Monoclonal Antibody (CPTC-CA8-2). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

References

Shimobayashi, E, et al. Molecular Neurobiology., U.S. National Library of Medicine, Oct. 201

NCBI and Uniprot Product Information

• NCBI GI #: 1012443759
• NCBI GeneID: 767
• NCBI Accession #: NP_001308766.1
• NCBI GenBank Nucleotide #: NM_001321837.1
• UniProt Accession #: P35219; Q32MY2; A8K0A5; B3KQZ7
• Molecular Weight: 32.97kDa
• NCBI Official Full Name: carbonic anhydrase-related protein isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase 8
• NCBI Official Symbol: CA8
• NCBI Official Synonym Symbols: CALS; CARP; CA-RP; CAMRQ3; CA-VIII
• NCBI Protein Information: carbonic anhydrase-related protein
• UniProt Protein Name: Carbonic anhydrase-related protein
• Protein Family: Carbonic anhydrase-related protein
• UniProt Gene Name: CA8
• UniProt Synonym Gene Names: CALS; CARP; CA-VIII

NCBI Description

The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Does not have a carbonic anhydrase catalytic activity.CautionAlthough it belongs to the alpha-carbonic anhydrase family, Arg-116 is present instead of the conserved His which is a zinc-binding residue. It is therefore expected that this protein lacks carbonic anhydrase activity.