Rabbit anti-Human, Mouse ROCK-1 Polyclonal Antibody | anti-ROCK-1 antibody

ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1, ROCK1, MGC131603, MGC43611, NY-REN-35 Antigen, p160 ROCK1, p160ROCK, Renal Carcinoma Antigen NY-REN-35, Rho-associated Protein Kinase 1) (HRP)

Cat. Num. AAA6498108

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: ROCK-1; Polyclonal Antibody; ROCK-1 (Rho-associated Coiled-coil-containing Protein Kinase 1; ROCK1; MGC131603; MGC43611; NY-REN-35 Antigen; p160 ROCK1; p160ROCK; Renal Carcinoma Antigen NY-REN-35; Rho-associated Protein Kinase 1) (HRP); anti-ROCK-1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ROCK-1. Species Crossreactiviy: mouse.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-ROCK-1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human ROCK-1.
• Conjugate: HRP
• Positive Control: A431 cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western Blot Analysis: Representative lot data. A431 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-ROCK-1 (1:500 dilution). Proteins were visualized using a Donkey Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system. Arrow indicates ROCK-1 (~ 158 kDa).)

Product Categories/Family for anti-ROCK-1 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4885583
• NCBI GeneID: 6093
• NCBI Accession #: NP_005397.1
• NCBI GenBank Nucleotide #: NM_005406.2
• UniProt Accession #: Q13464; Q2KHM4; Q59GZ4; B0YJ91
• Molecular Weight: 1354
• NCBI Official Full Name: rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1; p160-ROCK; Rho kinase; p160 ROCK-1; renal carcinoma antigen NY-REN-35; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I
• UniProt Protein Name: Rho-associated protein kinase 1
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_HUMAN

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; AGC group; DMPK family; ROCK subfamily

Chromosomal Location of Human Ortholog: 18q11.1

Cellular Component: Golgi membrane; centriole; ruffle; cytoskeleton; lamellipodium; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; GTP-Rho binding; ATP binding; protein kinase activity

Biological Process: regulation of cell adhesion; axon guidance; apoptosis; bleb formation; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; leukocyte adhesion; smooth muscle contraction; positive regulation of focal adhesion formation; regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; membrane to membrane docking; myoblast migration; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative regulation of neuron apoptosis; vascular endothelial growth factor receptor signaling pathway; actin cytoskeleton organization and biogenesis; leukocyte tethering or rolling; regulation of keratinocyte differentiation; leukocyte migration; cell structure disassembly during apoptosis