Western Blot (WB)
(Western Blot analysis of extracts of various cells, using ROBO4 antibody.)
Rabbit anti-Human ROBO4 Polyclonal Antibody | anti-ROBO4 antibody
ROBO4 Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
ROBO4; Polyclonal Antibody; ROBO4 Polyclonal Antibody; Roundabout homolog 4; Magic roundabout; anti-ROBO4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ROBO4 polyclonal antibody detects endogenous levels of ROBO4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
862
Applicable Applications for anti-ROBO4 antibody
Western Blot (WB)
Application Notes
WB: 1:200-1:500
Immunogen
Recombinant protein of human ROBO4.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using ROBO4 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using ROBO4 antibody.)
Related Product Information for anti-ROBO4 antibody
Secreted leucine-rich repeat-containing proteins 1 through 3 (Slit1-3) are secreted glycoproteins that influence axonal guidance and mediate normal neural progression by acting as high-affinity signaling ligands for the repulsive guidance receptors, robo1 and robo2 (also designated roundabout 1 and 2). Interactions between the robo receptor and Slit ligand families of proteins initiate signaling cascades that repel axonal outgrowth. The arrangement of the extracellular domains of robo4 diverges significantly from that of all other robo family members. Robo4 is the only robo family member expressed in primary endothelial cells. It binds Slit and inhibits cellular migration in a heterologous expression system. Together, the robo proteins prescribe developmental paths during neural development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 130kDa
NCBI Official Full Name
roundabout homolog 4 isoform 2
UniProt Protein Name
Roundabout homolog 4
Protein Family
UniProt Gene Name
ROBO4
UniProt Entry Name
ROBO4_HUMAN
Uniprot Description
ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: external side of plasma membrane; integral to membrane
Molecular Function: receptor activity
Biological Process: angiogenesis; cell differentiation; negative regulation of cell migration; regulation of apoptosis; regulation of cell migration
Similar Products
Product Notes
The ROBO4 robo4 (Catalog #AAA3009329) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROBO4 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ROBO4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:200-1:500. Researchers should empirically determine the suitability of the ROBO4 robo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROBO4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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