Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/25.)
Rabbit anti-Human ROBO4 Polyclonal Antibody | anti-ROBO4 antibody
ROBO4 Antibody
Gene Names
ROBO4; MRB; ECSM4
Reactivity
Human
Applications
Immunohistochemistry
Purity
Antigen affinity purification.
Synonyms
ROBO4; Polyclonal Antibody; ROBO4 Antibody; MRB; ECSM4; anti-ROBO4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ROBO4 protein.
Purity/Purification
Antigen affinity purification.
Form/Format
Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
Concentration
1.4 mg/ml (varies by lot)
Sequence Length
862
Applicable Applications for anti-ROBO4 antibody
Immunohistochemistry (IHC)
Application Notes
Immunohistochemistry: 1:25-1:100
Immunogen Type
Protein
Immunogen Description
Fusion protein corresponding to residues near the C terminal of human roundabout, axon guidance receptor, homolog 4 (Drosophila)
Target Name
ROBO4
Preparation and Storage
Store at -20 degree C
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/25.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Human brain tissue using at dilution 1/25.)
Related Product Information for anti-ROBO4 antibody
Robo4 (roundabout homolog 4 or magic roundabout) is a receptor for Slit proteins which implicate in angiogenesis and vascular patterning especially through facilitating inhibition of primary endothelial cell migration. It is a fairly recently identified member of Robo family and is shorter than other Robo members at both intracellular as well as extracellular domains. Robo4 plays important roles in several aspects of vascular development such as the guidance of endothelial cell migration, cell cycle regulation, inhibition of pathologic angiogenesis and endothelial hyperpermeability etc. Employing mouse and zebrafish models, Slit2/Robo4 interactions have been shown to maintain integrity of vascular network and its barrier function by inhibiting cytokine-mediated vasculogenesis with enhanced permeability, and that Slit2-Robo4-paxillin-GIT1 network inhibits neovascularization and vascular leakage. Moreover, single nucleotide polymorphorism of Robo4 has been documented to be associated with autism suggesting its role in development and function of human brain.
Product Categories/Family for anti-ROBO4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,282 Da
NCBI Official Full Name
roundabout homolog 4 isoform 2
NCBI Official Synonym Full Names
roundabout guidance receptor 4
NCBI Official Symbol
ROBO4
NCBI Official Synonym Symbols
MRB; ECSM4
NCBI Protein Information
roundabout homolog 4
UniProt Protein Name
Roundabout homolog 4
Protein Family
UniProt Gene Name
ROBO4
UniProt Entry Name
ROBO4_HUMAN
Uniprot Description
ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q24.2
Cellular Component: integral to membrane; external side of plasma membrane
Molecular Function: receptor activity
Biological Process: angiogenesis; cell differentiation; regulation of cell migration; negative regulation of cell migration
Research Articles on ROBO4
Similar Products
Product Notes
The ROBO4 robo4 (Catalog #AAA9402088) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ROBO4 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ROBO4 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Immunohistochemistry: 1:25-1:100. Researchers should empirically determine the suitability of the ROBO4 robo4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROBO4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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