ROBO4 recombinant protein

ROBO4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121028

• Gene Names: ROBO4; MRB; ECSM4
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ROBO4; ROBO4 Protein; Human; Recombinant (His Tag); Human ROBO4 Protein (His Tag); ECSM4 Protein; MRB Protein; roundabout; axon guidance receptor; homolog 4 (Drosophila); ROBO4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 5% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu469

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human ROBO4 (Q8WZ75-1) N-terminal four domains (Met 1-Glu 469) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ROBO4 recombinant protein

Background: Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.

References

Huminiecki L., et al., 2002, Genomics 79:547-552. Park,K.W. et al., 2003,Dev Biol. 261 (1):251-67. Yoshikawa,M. et al., 2008, Protein Expr Purif. 61 (1):78-82. Jones,C.A. et al., 2008, Nat Med. 14 (4):448-53. Koch,A.W. et al., 2011, Dev Cell. 20 (1):33-46.

NCBI and Uniprot Product Information

• NCBI GI #: 666637980
• NCBI GeneID: 54538
• NCBI Accession #: NP_001288017.1
• NCBI GenBank Nucleotide #: NM_001301088.1
• UniProt Accession #: Q8WZ75; Q14DU7; Q8TEG1; Q96JV6; Q9H718; Q9NWJ8; A8K154
• Molecular Weight: 75,282 Da
• NCBI Official Full Name: roundabout homolog 4 isoform 2
• NCBI Official Synonym Full Names: roundabout guidance receptor 4
• NCBI Official Symbol: ROBO4
• NCBI Official Synonym Symbols: MRB; ECSM4
• NCBI Protein Information: roundabout homolog 4
• UniProt Protein Name: Roundabout homolog 4
• Protein Family: Roundabout
• UniProt Gene Name: ROBO4
• UniProt Entry Name: ROBO4_HUMAN

Uniprot Description

ROBO4: Receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. May mediate the inhibition of primary endothelial cell migration by Slit proteins. Belongs to the immunoglobulin superfamily. ROBO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: external side of plasma membrane; integral to membrane

Molecular Function: receptor activity

Biological Process: angiogenesis; cell differentiation; negative regulation of cell migration; regulation of apoptosis; regulation of cell migration