Rabbit anti-Human EIF2S3L Polyclonal Antibody | anti-EIF2S3L antibody

EIF2S3L Antibody (Center)

Cat. Num. AAA9214289

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: EIF2S3L; Polyclonal Antibody; EIF2S3L Antibody (Center); Putative eukaryotic translation initiation factor 2 subunit 3-like protein; Eukaryotic translation initiation factor 2 subunit gamma A; eIF-2-gamma A; eIF-2gA; anti-EIF2S3L antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This EIF2S3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 273-299 amino acids from the Central region of human EIF2S3L.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 273-299
• Sequence Length: 466

• Applicable Applications for anti-EIF2S3L antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(EIF2S3L Antibody (Center) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EIF2S3L antibody detected the EIF2S3L protein (arrow).)

Related Product Information for anti-EIF2S3L antibody

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 205830883
• UniProt Accession #: Q2VIR3; Q5I0X0; Q6KF84
• Molecular Weight: 51229
• NCBI Official Full Name: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• UniProt Protein Name: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• Protein Family: Putative eukaryotic translation initiation factor 2 subunit 3-like protein
• UniProt Gene Name: EIF2S3L
• UniProt Synonym Gene Names: eIF-2-gamma A; eIF-2gA
• UniProt Entry Name: IF2GL_HUMAN

Uniprot Description

As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage ().