Western Blot (WB)
(Host: RabbitTarget Name: TOP3BSample Tissue: Human 293T Whole Cell lysatesAntibody Dilution: 1ug/ml)
Rabbit anti-Human TOP3B Polyclonal Antibody | anti-TOP3B antibody
TOP3B Antibody - middle region
Western Blot (WB)
(Host: RabbitTarget Name: TOP3BSample Tissue: Human 293T Whole Cell lysatesAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: TOP3BSample Tissue: Human 293T Whole Cell lysatesAntibody Dilution: 1ug/ml)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
Uniprot Description
TOP3B: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity. Belongs to the type IA topoisomerase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 5.99.1.2; DNA-binding; Topoisomerase
Chromosomal Location of Human Ortholog: 22q11.22
Cellular Component: nucleoplasm; condensed chromosome; nucleus
Molecular Function: DNA binding; DNA topoisomerase activity; DNA topoisomerase type I activity
Biological Process: DNA topological change; chromosome segregation
Research Articles on TOP3B
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Product Notes
The TOP3B top3b (Catalog #AAA3222764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP3B Antibody - middle region reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOP3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TOP3B top3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: YWADTVKRLL AEGINRPRKG HDAGDHPPIT PMKSATEAEL GGDAWRLYEY. It is sometimes possible for the material contained within the vial of "TOP3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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