Western Blot (WB)
(staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Goat RNF3, 247aa Isoform Polyclonal Antibody
RNF3, 247aa Isoform (Polycomb Group RING Finger Protein 3, RING Finger Protein 3A, PCGF3, RNF3A, MGC40413)
Reactivity
Bovine, Canine, Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
RNF3; 247aa Isoform; Polyclonal Antibody; 247aa Isoform (Polycomb Group RING Finger Protein 3; RING Finger Protein 3A; PCGF3; RNF3A; MGC40413); Anti -RNF3; anti-RNF3; 247aa Isoform antibody
Host
Goat
Reactivity
Bovine, Canine, Human, Mouse
Clonality
Polyclonal
Specificity
Recognizes human RNF3. Species sequence homology: Bovine, canine and mouse.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Applicable Applications for anti-RNF3, 247aa Isoform antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:2000
Western Blot: 0.5-2ug/ml, human heart lysate observed on ~35kD bands
Dilution: ELISA: 1:2000
Western Blot: 0.5-2ug/ml, human heart lysate observed on ~35kD bands
Immunogen
Synthetic peptide corresponding to C-PLLLHYRPKMDLL, from human RNF3, at C-terminal (NP_006306.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB)
(staining (1ug/ml) of Human Heart lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Related Product Information for anti-RNF3, 247aa Isoform antibody
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Product Categories/Family for anti-RNF3, 247aa Isoform antibody
Similar Products
Product Notes
The RNF3, 247aa Isoform (Catalog #AAA6004775) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The RNF3, 247aa Isoform (Polycomb Group RING Finger Protein 3, RING Finger Protein 3A, PCGF3, RNF3A, MGC40413) reacts with Bovine, Canine, Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RNF3, 247aa Isoform can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:2000 Western Blot: 0.5-2ug/ml, human heart lysate observed on ~35kD bands. Researchers should empirically determine the suitability of the RNF3, 247aa Isoform for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNF3, 247aa Isoform, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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