Human Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit | NEFH elisa kit

Human Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit

Cat. Num. AAA456113

• Gene Names: NEFH; NFH; CMT2CC
• Reactivity: Human
• Synonyms: Neurofilament; Heavy Polypeptide (NEFH); Human Neurofilament; Heavy Polypeptide (NEFH) ELISA Kit; NEFH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NEFH. No significant cross-reactivity or interference between NEFH and analogues was observed.
• Sequence Length: 963

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.2pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEFH were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEFH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NEFH elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of NEFH in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEFH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NEFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NEFH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32483416
• NCBI GeneID: 4744
• NCBI Accession #: NP_066554.2
• NCBI GenBank Nucleotide #: NM_021076.3
• UniProt Accession #: P12036; Q96HF8; Q9UJS7; Q9UQ14; B4DYY4
• Molecular Weight: 105,639 Da
• NCBI Official Full Name: neurofilament heavy polypeptide
• NCBI Official Synonym Full Names: neurofilament heavy
• NCBI Official Symbol: NEFH
• NCBI Official Synonym Symbols: NFH; CMT2CC
• NCBI Protein Information: neurofilament heavy polypeptide
• UniProt Protein Name: Neurofilament heavy polypeptide
• Protein Family: Neurofilament heavy polypeptide
• UniProt Gene Name: NEFH
• UniProt Synonym Gene Names: KIAA0845; NFH; NF-H

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.