Rabbit anti-Human NR0B1 Polyclonal Antibody | anti-NR0B1 antibody

NR0B1, ID (NR0B1, AHC, DAX1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1) (FITC)

Cat. Num. AAA6326460

• Gene Names: NR0B1; AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NR0B1; Polyclonal Antibody; ID (NR0B1; AHC; DAX1; Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1) (FITC); Nuclear receptor DAX-1; anti-NR0B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NR0B1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NR0B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-333 amino acids from the Central region of human NR0B1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NR0B1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NR0B1 antibody detected the NR0B1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of NR0B1 (arrow) using rabbit polyclonal NR0B1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NR0B1 gene.)

Immunohistochemistry (IHC)

(NR0B1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR0B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NR0B1 antibody

This gene encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Product Categories/Family for anti-NR0B1 antibody

Antibodies; Receptors

References

Li, N., et al. J. Clin. Endocrinol. Metab. 95 (9), E104-E111 (2010): Nedumaran, B., et al. J. Biol. Chem. 285(12):9221-9232(2010). Kinsey, M., et al. Cancer Res. 69(23):9047-9055(2009). Nagl, F., et al. Am. J. Physiol., Cell Physiol. 297 (5), C1146-C1156 (2009): Skinningsrud, B., et al. J. Clin. Endocrinol. Metab. 94(10):4086-4093(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 5016090
• NCBI GeneID: 190
• NCBI Accession #: NP_000466.2
• NCBI GenBank Nucleotide #: NM_000475.4
• UniProt Accession #: P51843; Q96F69
• Molecular Weight: 51,718 Da
• NCBI Official Full Name: nuclear receptor subfamily 0 group B member 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 0, group B, member 1
• NCBI Official Symbol: NR0B1
• NCBI Official Synonym Symbols: AHC; AHX; DSS; GTD; HHG; AHCH; DAX1; DAX-1; NROB1; SRXY2
• NCBI Protein Information: nuclear receptor subfamily 0 group B member 1; nuclear receptor DAX1; nuclear receptor DAX-1; nuclear hormone receptor; DSS-AHC critical region on the X chromosome protein 1
• UniProt Protein Name: Nuclear receptor subfamily 0 group B member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR0B1
• UniProt Synonym Gene Names: AHC; DAX1
• UniProt Entry Name: NR0B1_HUMAN

Uniprot Description

NR0B1: orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. Homodimerizes with STF-1, NR5A2, NR0B2 and with COPS2. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus. Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with STF-1 and NROB2 through its N-terminal LXXLL motifs. Defects in NR0B1 are the cause of congenital X-linked adrenal hypoplasia. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: Xp21.3

Cellular Component: nucleoplasm; polysomal ribosome; membrane; cytoplasm; nucleus

Molecular Function: protein domain specific binding; DNA hairpin binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; AF-2 domain binding; RNA binding; sequence-specific DNA binding; steroid hormone receptor activity; transcription corepressor activity; transcription factor binding; steroid hormone receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; hypothalamus development; negative regulation of steroid hormone receptor signaling pathway; intracellular receptor-mediated signaling pathway; gonad development; adrenal gland development; negative regulation of transcription factor activity; male gonad development; Sertoli cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell differentiation; protein localization; Leydig cell differentiation; pituitary gland development; male sex determination; steroid hormone mediated signaling; spermatogenesis; gene expression; negative regulation of transcription, DNA-dependent; steroid biosynthetic process

Disease: 46,xy Sex Reversal 2; Adrenal Hypoplasia, Congenital