Rabbit anti-Human FFAR1 Polyclonal Antibody | anti-FFAR1 antibody

FFAR1, CT (FFAR1, GPR40, Free fatty acid receptor 1, G-protein coupled receptor 40) (Azide free) (HRP)

Cat. Num. AAA6298883

• Gene Names: FFAR1; FFA1R; GPR40; GPCR40
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FFAR1; Polyclonal Antibody; CT (FFAR1; GPR40; Free fatty acid receptor 1; G-protein coupled receptor 40) (Azide free) (HRP); G-protein coupled receptor 40; anti-FFAR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FFAR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FFAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 280-300 amino acids from the C-terminal region of human FFAR1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FFAR1 Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the FFAR1 antibody detected the FFAR1 protein (arrow).)

Related Product Information for anti-FFAR1 antibody

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.

Product Categories/Family for anti-FFAR1 antibody

Antibodies; G Protein Receptors

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Lu, S.Y., et al. J. Mol. Graph. Model. 28(8):766-774(2010). Del Guerra, S., et al. Nutr Metab Cardiovasc Dis 20(1):22-25(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Nagasumi, K., et al. Diabetes 58(5):1067-1076(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4885327
• NCBI GeneID: 2864
• NCBI Accession #: NP_005294.1
• NCBI GenBank Nucleotide #: NM_005303.2
• UniProt Accession #: O14842; Q0VAS2; Q4VBL4
• Molecular Weight: 31,457 Da
• NCBI Official Full Name: free fatty acid receptor 1
• NCBI Official Synonym Full Names: free fatty acid receptor 1
• NCBI Official Symbol: FFAR1
• NCBI Official Synonym Symbols: FFA1R; GPR40; GPCR40
• NCBI Protein Information: free fatty acid receptor 1; G protein-coupled receptor 40; G-protein coupled receptor 40
• UniProt Protein Name: Free fatty acid receptor 1
• Protein Family: Free fatty acid receptor
• UniProt Gene Name: FFAR1
• UniProt Synonym Gene Names: GPR40
• UniProt Entry Name: FFAR1_HUMAN

NCBI Description

This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]

Uniprot Description

FFAR1: Receptor for medium and long chain saturated and unsaturated fatty acids. Binding of the ligand increase intracellular calcium concentration and amplify glucose-stimulated insulin secretion. The activity of this receptor is mediated by G- proteins that activate phospholipase C. Seems to act through a G(q) and G(i)-mediated pathway. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity; lipid binding; bioactive lipid receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; insulin secretion; energy reserve metabolic process; glucose homeostasis; positive regulation of calcium ion transport; regulation of insulin secretion; positive regulation of GTPase activity