Rabbit RNF144B Polyclonal Antibody | anti-RNF144B antibody

RNF144B polyclonal antibody

Cat. Num. AAA3014814

• Gene Names: RNF144B; PIR2; IBRDC2; p53RFP; bA528A10.3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: RNF144B; Polyclonal Antibody; RNF144B polyclonal antibody; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein; IBRDC2; P53RFP; anti-RNF144B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: RNF144B polyclonal antibody detects endogenous levels of RNF144B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RNF144B antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human RNF144B
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of RNF144B polyclonal antibody at 1:500 dilutionLaneA: H1792 whole cell lysateLaneB: A549 whole cell lysateLaneC: CT26 whole cell lysateLaneD: PC12 whole cell lysate)

Related Product Information for anti-RNF144B antibody

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.

NCBI and Uniprot Product Information

• NCBI GI #: 50284696
• NCBI GeneID: 255488
• NCBI Accession #: NP_877434.2
• NCBI GenBank Nucleotide #: NM_182757.3
• UniProt Accession #: Q7Z419; Q5TB85; Q6P4Q0; Q8N3R7; Q9BX38; B3KUA8
• Molecular Weight: 33,697 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF144B
• NCBI Official Synonym Full Names: ring finger protein 144B
• NCBI Official Symbol: RNF144B
• NCBI Official Synonym Symbols: PIR2; IBRDC2; p53RFP; bA528A10.3
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF144B; IBR domain containing 2; IBR domain-containing protein 2; p53-inducible RING finger protein
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF144B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF144B
• UniProt Synonym Gene Names: IBRDC2; P53RFP
• UniProt Entry Name: R144B_HUMAN

Uniprot Description

RNF144B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. Belongs to the RBR family. RNF144 subfamily.

Protein type: Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Ligase

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; mitochondrial membrane; integral to membrane; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process