Rabbit anti-Human RNF144B Polyclonal Antibody | anti-RNF144B antibody

RNF144B Antibody

Cat. Num. AAA9405507

• Gene Names: RNF144B; PIR2; IBRDC2; p53RFP; bA528A10.3
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: RNF144B; Polyclonal Antibody; RNF144B Antibody; PIR2; IBRDC2; p53RFP; bA528A10.3; anti-RNF144B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total RNF144B protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 2.4 mg/ml (varies by lot)
• Sequence Length: 303

• Applicable Applications for anti-RNF144B antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50-1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to residues near the N terminal of human ring finger protein 144B
• Target Name: RNF144B
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/50.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue using at dilution 1/50.)

Related Product Information for anti-RNF144B antibody

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.

Product Categories/Family for anti-RNF144B antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 50284696
• NCBI GeneID: 255488
• NCBI Accession #: NP_877434.2
• NCBI GenBank Nucleotide #: NM_182757.3
• UniProt Accession #: Q7Z419; Q5TB85; Q6P4Q0; Q8N3R7; Q9BX38; B3KUA8; B4DZI2
• Molecular Weight: 23,856 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF144B
• NCBI Official Synonym Full Names: ring finger protein 144B
• NCBI Official Symbol: RNF144B
• NCBI Official Synonym Symbols: PIR2; IBRDC2; p53RFP; bA528A10.3
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF144B
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF144B
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF144B
• UniProt Synonym Gene Names: IBRDC2; P53RFP
• UniProt Entry Name: R144B_HUMAN

Uniprot Description

RNF144B: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. Belongs to the RBR family. RNF144 subfamily.

Protein type: Membrane protein, integral; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: mitochondrial membrane; cytoplasm; integral to membrane; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: apoptosis; protein ubiquitination during ubiquitin-dependent protein catabolic process