Goat TRXR1 / TXNRD1 Polyclonal Antibody | anti-TRXR1 antibody

Goat Polyclonal to Human TRXR1 / TXNRD1

Cat. Num. AAA244270

• Gene Names: TXNRD1; TR; TR1; TXNR; TRXR1; GRIM-12
• Reactivity: Gibbon, Dog, Gorilla, Human, Monkey, Orangutan, Pig; Predicted Reactivity: Bat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRXR1 / TXNRD1; Polyclonal Antibody; Goat Polyclonal to Human TRXR1 / TXNRD1; Anti-TRXR1 / TXNRD1 Antibody (C-Terminus) IHC-plus; TXNRD1; GRIM12; KDRF; Oxidoreductase; TRXR1; Thioredoxin reductase GRIM-12; Thioredoxin reductase 1; TR; GRIM-12; Thioredoxin reductase; Thioredoxin reductase TR1; TR1; TXNR; Human TRXR1; anti-TRXR1 antibody

• Host: Goat
• Reactivity: Gibbon, Dog, Gorilla, Human, Monkey, Orangutan, Pig; Predicted Reactivity: Bat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human TXNRD1. This antibody is expected to recognise reported isoform 1 (NP_003321.2), isoform 2 (NP_877393.1; NP_877419.1; NP_877420.1) and isoform 3 (NP_001087240.1)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-TRXR1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (2.5 ug/ml), WB (1:32000, 0.1 - 1 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide TKRSGASILQAGC from the C-terminus of human TXNRD1 (NP_003321.2; NP_877393.1; NP_877419.1; NP_877420.1; NP_001087240.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Dog, Pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: TRXR1 / TXNRD1 antibody was raised against synthetic peptide TKRSGASILQAGC from the C-terminus of human TXNRD1 (NP_003321.2; NP_877393.1; NP_877419.1; NP_877420.1; NP_001087240.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Dog, Pig (100%); Elephant, Bat, Opossum (92%); Rabbit (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TXNRD1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-TXNRD1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Antibody staining (0.1 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 148277065
• NCBI GeneID: 7296
• NCBI Accession #: NP_003321.3
• NCBI GenBank Nucleotide #: NM_003330.3
• UniProt Accession #: Q16881; Q6FI31; Q6VB40; Q6VB41; Q6VB42; Q6VBP2; Q6VBP3; B7Z1F4; B7Z3Y8; B7Z904; E9PMY9; F5H780
• Molecular Weight: 50,873 Da
• NCBI Official Full Name: thioredoxin reductase 1, cytoplasmic isoform 1
• NCBI Official Synonym Full Names: thioredoxin reductase 1
• NCBI Official Symbol: TXNRD1
• NCBI Official Synonym Symbols: TR; TR1; TXNR; TRXR1; GRIM-12
• NCBI Protein Information: thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein
• UniProt Protein Name: Thioredoxin reductase 1, cytoplasmic
• UniProt Gene Name: TXNRD1
• UniProt Synonym Gene Names: GRIM12; KDRF; TR; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein
• UniProt Entry Name: TRXR1_HUMAN

NCBI Description

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.

Protein type: Oxidoreductase; EC 1.8.1.9; Nucleotide Metabolism - pyrimidine; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: nucleoplasm; nucleolus; cytosol

Molecular Function: protein binding; thioredoxin-disulfide reductase activity; electron carrier activity; FAD binding; protein disulfide oxidoreductase activity

Biological Process: response to reactive oxygen species; cell redox homeostasis; nucleobase, nucleoside and nucleotide interconversion; nucleobase, nucleoside and nucleotide metabolic process; cellular lipid metabolic process; signal transduction