Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF144A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Rabbit anti-Human, Rat RNF144A Polyclonal Antibody | anti-RNF144A antibody
RNF144A Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF144A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RNF144A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this protein contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. The mouse counterpart of this protein has been shown to interact with Ube2l3/UbcM4, which is an ubiquitin-conjugating enzyme involved in embryonic development. [provided by RefSeq, Jul 2008]
Uniprot Description
RNF144A: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Belongs to the RBR family. RNF144 subfamily.
Protein type: Ligase; EC 6.3.2.-; Membrane protein, integral; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 2p25.2
Cellular Component: Golgi apparatus; cytoplasmic vesicle membrane; plasma membrane; integral to membrane
Molecular Function: zinc ion binding; ligase activity
Biological Process: protein ubiquitination
Research Articles on RNF144A
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Product Notes
The RNF144A rnf144a (Catalog #AAA9142866) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNF144A Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RNF144A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RNF144A rnf144a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ELLIKEGLET AISCPDAACP KQGHLQENEI ECMVAAEIMQ RYKKLQFERE VLFDPCRTWC PASTCQAVCQ LQDVGLQTPQ PVQCKACRME FCSTCKASWH PGQGCPETMP ITFLPGETSA AFKMEEDD. It is sometimes possible for the material contained within the vial of "RNF144A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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