Rabbit anti-Human RIP140 Polyclonal Antibody | anti-RIP140 antibody

RIP140, ID (NRIP1, Nuclear receptor-interacting protein 1, Nuclear factor RIP140, Receptor-interacting protein 140) (PE)

Cat. Num. AAA6342180

• Gene Names: NRIP1; RIP140
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RIP140; Polyclonal Antibody; ID (NRIP1; Nuclear receptor-interacting protein 1; Nuclear factor RIP140; Receptor-interacting protein 140) (PE); NRIP1; Receptor-interacting protein 140; anti-RIP140 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RIP140 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RIP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585-613 amino acids from the Central region of human RIP140.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RIP140 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RIP140 antibody detected the RIP140 protein (arrow).)

Related Product Information for anti-RIP140 antibody

Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor.

Product Categories/Family for anti-RIP140 antibody

Antibodies; Receptors

References

Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010). Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Docquier, A., et al. Clin. Cancer Res. 16(11):2959-2970(2010). Fritah, A., et al. Cardiovasc. Res. 86(3):443-451(2010). Suzuki, A., et al. J. Biol. Chem. 285(18):13444-13453(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 57232746
• NCBI GeneID: 8204
• NCBI Accession #: NP_003480.2
• NCBI GenBank Nucleotide #: NM_003489.3
• UniProt Accession #: P48552; Q8IWE8
• Molecular Weight: 126,942 Da
• NCBI Official Full Name: nuclear receptor-interacting protein 1
• NCBI Official Synonym Full Names: nuclear receptor interacting protein 1
• NCBI Official Symbol: NRIP1
• NCBI Official Synonym Symbols: RIP140
• NCBI Protein Information: nuclear receptor-interacting protein 1; nuclear factor RIP140; receptor interacting protein 140; receptor-interacting protein 140
• UniProt Protein Name: Nuclear receptor-interacting protein 1
• UniProt Gene Name: NRIP1
• UniProt Entry Name: NRIP1_HUMAN

NCBI Description

Nuclear receptor interacting protein 1 (NRIP1) is a nuclear protein that specifically interacts with the hormone-dependent activation domain AF2 of nuclear receptors. Also known as RIP140, this protein modulates transcriptional activity of the estrogen receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

RIP140: Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Interacts with RARA and RXRB homodimers and RARA/RXRB heterodimers in the presence of ligand. Interacts with HDAC1 and HDAC3 via its N-terminal domain. Interacts with NR2C1 (sumoylated form and via the ligand-binding domain); the interaction results in promoting the repressor activity of NR2C1. Interacts with CTBP1, CTBP2, ESR1, HDAC1, HDAC2, HDAC5, HDAC6, NR2C2, NR3C1, NR3C2, YWHAH, JUN and FOS. Found in a complex with both NR3C1 and YWHAH. Interacts with ZNF366.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 21q11.2

Cellular Component: nucleoplasm; histone deacetylase complex; nucleolus; nuclear speck; nucleus; cell junction

Molecular Function: retinoid X receptor binding; protein binding; androgen receptor binding; histone deacetylase binding; transcription coactivator activity; estrogen receptor binding; transcription corepressor activity; glucocorticoid receptor binding; nuclear hormone receptor binding

Biological Process: circadian rhythm; sequestering of lipid; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; negative regulation of transcription from RNA polymerase II promoter; ovarian follicle rupture