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Immunofluorescence (IF) (Confocal immunofluorescent analysis of TUBB2B Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit anti-Human TUBB2B Polyclonal Antibody | anti-TUBB2B antibody

TUBB2B, NT (TUBB2B, Tubulin beta-2B chain) (APC)

Gene Names
TUBB2B; PMGYSA; bA506K6.1
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TUBB2B; Polyclonal Antibody; NT (TUBB2B; Tubulin beta-2B chain) (APC); Tubulin beta-2B chain; anti-TUBB2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-TUBB2B antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IF: 1:10-50
FLISA: 1:1,000
IHC: 1:10-50
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-47 amino acids from the N-terminal region of human TUBB2B.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of TUBB2B Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF) (Confocal immunofluorescent analysis of TUBB2B Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Western Blot (WB)

(TUBB2B Antibody (N-term) western blot analysis in HepG2,Hela,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TUBB2B antibody detected the TUBB2B protein (arrow).)

Western Blot (WB) (TUBB2B Antibody (N-term) western blot analysis in HepG2,Hela,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TUBB2B antibody detected the TUBB2B protein (arrow).)

Immunohistochemistry (IHC)

(TUBB2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (TUBB2B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TUBB2B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TUBB2B antibody
The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq].
Product Categories/Family for anti-TUBB2B antibody
References
Xu, W., et al. Mol. Cancer Ther. 8(12):3318-3330(2009). Jaglin, X.H., et al. Nat. Genet. 41(6):746-752(2009). Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009). Cucchiarelli, V., et al. Cell Motil. Cytoskeleton 65(8):675-685(2008). Lamesch, P., et al. Genomics 89(3):307-315(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49,953 Da
NCBI Official Full Name
tubulin beta-2B chain
NCBI Official Synonym Full Names
tubulin, beta 2B class IIb
NCBI Official Symbol
TUBB2B
NCBI Official Synonym Symbols
PMGYSA; bA506K6.1
NCBI Protein Information
tubulin beta-2B chain; class II beta-tubulin isotype; class IIb beta-tubulin; tubulin, beta polypeptide paralog
UniProt Protein Name
Tubulin beta-2B chain
Protein Family
UniProt Gene Name
TUBB2B

NCBI Description

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq, Mar 2010]

Uniprot Description

Tubulin is the major constituent of microtubules (PubMed:23001566, PubMed:28013290, PubMed:26732629). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (). Plays a critical role in proper axon guidance in both central and peripheral axon tracts (PubMed:23001566). Implicated in neuronal migration (PubMed:19465910).

Research Articles on TUBB2B

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Product Notes

The TUBB2B tubb2b (Catalog #AAA6360146) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUBB2B, NT (TUBB2B, Tubulin beta-2B chain) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TUBB2B can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB). IF: 1:10-50 FLISA: 1:1,000 IHC: 1:10-50 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TUBB2B tubb2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBB2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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