Rabbit RICTOR Polyclonal Antibody | anti-RICTOR antibody

RICTOR Antibody

Cat. Num. AAA9608139

• Gene Names: RICTOR; PIA; AVO3; hAVO3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RICTOR; Polyclonal Antibody; RICTOR Antibody; AVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; Likely ortholog of mouse TORC2 specific protein AVO3 (S. cerevisiae); mAVO3; MGC39830; PIA; Pianissimo; Rapamycin insensitive companion of mTOR; Rapamycin-insensitive companion of mTOR; Rictor; RICTR; RICTR_HUMAN; RPTOR independent companion of MTOR complex 2; TORC2 specific protein AVO3; anti-RICTOR antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RICTOR antibody detects endogenous levels of total RICTOR
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1732

• Applicable Applications for anti-RICTOR antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IHC: 1:200
• Immunogen: A synthesized peptide derived from human RICTOR
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(MBS9608139 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of RICTOR using HUVEC whole cell lysates)

Related Product Information for anti-RICTOR antibody

Function: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Subunit Structure: Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR (PubMed:15268862, PubMed:15467718, PubMed:17461779, PubMed:17599906). Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to MTOR and PRR5 within the TORC2 complex (PubMed:15268862, PubMed:17461779, PubMed:17599906). Interacts with CCDC28B (PubMed:23727834). Interacts with NBN (PubMed:23762398). Interacts with PRR5L (PubMed:17461779, PubMed:21964062).
Post-translational Modifications: Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling.
Similarity: Belongs to the RICTOR family.

References

Wu D, Huang CJ, Jiao XF, Ding ZM, Zhang JY, Chen F, Wang YS, Li X, Huo LJ; Journal: Oncotarget. Olaquindox disrupts tight junction integrity and cytoskeleton architecture in mouse Sertoli cells.

NCBI and Uniprot Product Information

• NCBI GI #: 550544217
• NCBI GeneID: 253260
• NCBI Accession #: NP_001272368.1
• NCBI GenBank Nucleotide #: NM_001285439.1
• UniProt Accession #: Q6R327; Q68DT5; Q86UB7; Q8N3A0; Q8NCM6; B2RNX0; B7ZMF7
• Molecular Weight: Observed: 192 kDa; Predicted: 193 kDa
• NCBI Official Full Name: rapamycin-insensitive companion of mTOR isoform 2
• NCBI Official Synonym Full Names: RPTOR independent companion of MTOR complex 2
• NCBI Official Symbol: RICTOR
• NCBI Official Synonym Symbols: PIA; AVO3; hAVO3
• NCBI Protein Information: rapamycin-insensitive companion of mTOR
• UniProt Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Family: Rapamycin-insensitive companion
• UniProt Gene Name: RICTOR
• UniProt Synonym Gene Names: hAVO3

NCBI Description

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

Uniprot Description

Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.