Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat RICTOR Polyclonal Antibody | anti-RICTOR antibody
Goat anti-RICTOR Antibody
Immunohistochemistry: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml.
Western Blot: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]
Uniprot Description
RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.
Protein type: Adaptor/scaffold; Cell cycle regulation; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: cytosol; TORC2 complex
Molecular Function: enzyme activator activity; protein binding; protein kinase binding; ribosome binding
Biological Process: actin cytoskeleton reorganization; embryonic development; establishment and/or maintenance of actin cytoskeleton polarity; establishment of cell polarity; peptidyl-serine phosphorylation; phosphoinositide-mediated signaling; positive regulation of actin filament polymerization; positive regulation of catalytic activity; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of TOR signaling pathway; regulation of actin cytoskeleton organization and biogenesis; regulation of gene expression; regulation of GTPase activity; regulation of inflammatory response; regulation of peptidyl-serine phosphorylation; regulation of phosphorylation; regulation of protein kinase B signaling cascade; T cell costimulation; TOR signaling pathway
Research Articles on RICTOR
Similar Products
Product Notes
The RICTOR rictor (Catalog #AAA421193) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RICTOR Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's RICTOR can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded Kidney shows strong cytoplasmic staining of epithelial cells in distal convoluted tubules. Recommended concentration, 2-3ug/ml. Western Blot: Preliminary experiments gave an approx 38kDa band in Human Lung Carcinoma A549 cell line and cervix epitheloid carcinoma cell line HeLa lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 192kDa according to NP_689969.2. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Researchers should empirically determine the suitability of the RICTOR rictor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KQPIVDTSAE S. It is sometimes possible for the material contained within the vial of "RICTOR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
