Western Blot (WB)
(ECSIT Antibody (C-term) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).)
Rabbit anti-Human ECSIT Polyclonal Antibody | anti-ECSIT antibody
ECSIT, CT (ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC) (PE)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(ECSIT Antibody (C-term) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).)
Western Blot (WB)
(ECSIT Antibody (C-term) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with ECSIT Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ECSIT primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ECSIT immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NCBI and Uniprot Product Information
Uniprot Description
ECSIT: Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. Belongs to the ECSIT family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: nucleoplasm; transcription factor complex; mitochondrion; cytoplasm; nucleus
Molecular Function: signal transducer activity; protein binding; oxidoreductase activity, acting on NADH or NADPH; transcription factor activity
Biological Process: BMP signaling pathway; mesoderm formation; innate immune response; regulation of oxidoreductase activity
Research Articles on ECSIT
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Product Notes
The ECSIT ecsit (Catalog #AAA6294290) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECSIT, CT (ECSIT, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, Protein SITPEC) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ECSIT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ECSIT ecsit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECSIT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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