Rabbit anti-Human RHOG Polyclonal Antibody | anti-RHOG antibody

RHOG, CT (RHOG, ARHG, Rho-related GTP-binding protein RhoG)

Cat. Num. AAA6001225

• Gene Names: RHOG; ARHG
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RHOG; Polyclonal Antibody; CT (RHOG; ARHG; Rho-related GTP-binding protein RhoG); Anti -RHOG; anti-RHOG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RHOG antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: RHOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the C-terminal region of human RHOG.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RHOG Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RHOG antibody detected the RHOG protein (arrow).)

Western Blot (WB)

(Western blot analysis of RHOG (arrow) using rabbit polyclonal RHOG Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RHOG gene.)

Related Product Information for anti-RHOG antibody

ARHG is a member of the RAS superfamily of genes, which encode GTP-binding proteins that act in the pathway of signal transduction and play a key role in the regulation of cellular functions.

Product Categories/Family for anti-RHOG antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 46249393
• NCBI GeneID: 391
• NCBI Accession #: NP_001656.2
• NCBI GenBank Nucleotide #: NM_001665.3
• UniProt Accession #: P84095; P35238; Q8NI04
• Molecular Weight: 21,309 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoG
• NCBI Official Synonym Full Names: ras homolog family member G
• NCBI Official Symbol: RHOG
• NCBI Official Synonym Symbols: ARHG
• NCBI Protein Information: rho-related GTP-binding protein RhoG; ras homolog gene family, member G (rho G)
• UniProt Protein Name: Rho-related GTP-binding protein RhoG
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOG
• UniProt Synonym Gene Names: ARHG
• UniProt Entry Name: RHOG_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOG: Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: focal adhesion; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: axon guidance; platelet activation; regulation of small GTPase mediated signal transduction; metabolic process; positive regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; positive regulation of cell proliferation; Rac protein signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation; Rho protein signal transduction