Rabbit RHOG Polyclonal Antibody | anti-RHOG antibody

RHOG Antibody

Cat. Num. AAA153338

• Gene Names: RHOG; ARHG
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: RHOG antibody is affinity chromatography purified via peptide column.
• Synonyms: RHOG; Polyclonal Antibody; RHOG Antibody; Ras homolog family member G; Rho-related GTP-binding protein; ARHG ; ras homolog family member G; anti-RHOG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RHOG antibody is human, mouse and rat reactive.
• Purity/Purification: RHOG antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 191

• Applicable Applications for anti-RHOG antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
• Conjugate: Unconjugated
• Immunogen: RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG.
• Buffer: RHOG antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: RHOG antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.)

Immunohistochemistry (IHC)

(Immunohistochemistry of RHOG in mouse kidney tissue with RHOG antibody at 5 μg/ml.)

Related Product Information for anti-RHOG antibody

The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).

NCBI and Uniprot Product Information

• NCBI GI #: 46249393
• NCBI GeneID: 391
• NCBI Accession #: NP_001656
• NCBI GenBank Nucleotide #: NM_001665.3
• UniProt Accession #: P84095; P35238; Q8NI04
• Molecular Weight: Predicted: 21 kDa; ; Observed: 23 kDa
• NCBI Official Full Name: rho-related GTP-binding protein RhoG
• NCBI Official Synonym Full Names: ras homolog family member G
• NCBI Official Symbol: RHOG
• NCBI Official Synonym Symbols: ARHG
• NCBI Protein Information: rho-related GTP-binding protein RhoG; ras homolog gene family, member G (rho G)
• UniProt Protein Name: Rho-related GTP-binding protein RhoG
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOG
• UniProt Synonym Gene Names: ARHG
• UniProt Entry Name: RHOG_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOG: Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric, Rho; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: focal adhesion; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: axon guidance; platelet activation; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; Rac protein signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation; Rho protein signal transduction