Rabbit anti-Human RHOG Polyclonal Antibody | anti-RHOG antibody

RHOG, CT (RHOG, ARHG, Rho-related GTP-binding protein RhoG) (PE)

Cat. Num. AAA6341971

• Gene Names: RHOG; ARHG
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RHOG; Polyclonal Antibody; CT (RHOG; ARHG; Rho-related GTP-binding protein RhoG) (PE); Rho-related GTP-binding protein RhoG; anti-RHOG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-RHOG antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RHOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the C-terminal region of human RHOG.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RHOG Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the RHOG antibody detected the RHOG protein (arrow).)

Western Blot (WB)

(Western blot analysis of RHOG (arrow) using rabbit polyclonal RHOG Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RHOG gene.)

Related Product Information for anti-RHOG antibody

ARHG is a member of the RAS superfamily of genes, which encode GTP-binding proteins that act in the pathway of signal transduction and play a key role in the regulation of cellular functions.

Product Categories/Family for anti-RHOG antibody

Antibodies; Binding Proteins

References

Hiramoto-Yamaki, N., et al. J. Cell Biol. 190(3):461-477(2010). Segat, L., et al. Vaccine 28(10):2201-2206(2010). Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009): Roppenser, B., et al. J. Cell. Sci. 122 (PT 5), 696-705 (2009): van Buul, J.D., et al. J. Cell Biol. 178(7):1279-1293(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 46249393
• NCBI GeneID: 391
• NCBI Accession #: NP_001656.2
• NCBI GenBank Nucleotide #: NM_001665.3
• UniProt Accession #: P84095; P35238; Q8NI04
• Molecular Weight: 21,309 Da
• NCBI Official Full Name: rho-related GTP-binding protein RhoG
• NCBI Official Synonym Full Names: ras homolog family member G
• NCBI Official Symbol: RHOG
• NCBI Official Synonym Symbols: ARHG
• NCBI Protein Information: rho-related GTP-binding protein RhoG; ras homolog gene family, member G (rho G)
• UniProt Protein Name: Rho-related GTP-binding protein RhoG
• Protein Family: Rho-related GTP-binding protein
• UniProt Gene Name: RHOG
• UniProt Synonym Gene Names: ARHG
• UniProt Entry Name: RHOG_HUMAN

NCBI Description

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The encoded protein facilitates translocation of a functional guanine nucleotide exchange factor (GEF) complex from the cytoplasm to the plasma membrane where ras-related C3 botulinum toxin substrate 1 is activated to promote lamellipodium formation and cell migration. Two related pseudogene have been identified on chromosomes 20 and X. [provided by RefSeq, Aug 2011]

Uniprot Description

RHOG: Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. Belongs to the small GTPase superfamily. Rho family.

Protein type: G protein, monomeric; Motility/polarity/chemotaxis; G protein, monomeric, Rho

Chromosomal Location of Human Ortholog: 11p15.5-p15.4

Cellular Component: focal adhesion; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: axon guidance; platelet activation; regulation of small GTPase mediated signal transduction; metabolic process; small GTPase mediated signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; Rac protein signal transduction; actin cytoskeleton organization and biogenesis; blood coagulation; Rho protein signal transduction