Rabbit anti-Human, Rat RHOBTB3 Polyclonal Antibody | anti-RHOBTB3 antibody

RHOBTB3 (L365) Polyclonal Antibody

Cat. Num. AAA3013735

• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RHOBTB3; Polyclonal Antibody; RHOBTB3 (L365) Polyclonal Antibody; KIAA0878; Rho related BTB domain containing protein 3; RHOBTB 3; anti-RHOBTB3 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RHOBTB3 (L365) polyclonal antibody detects endogenous levels of RHOBTB3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 611

• Applicable Applications for anti-RHOBTB3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 341-390 of Human RHOBTB3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RHOBTB3 (L365) polyclonal antibody at 1:1000 dilution Lane1:HKE293T whole cell lysate (56ug) Lane2:MCF-7 whole cell lysate (48ug) Lane3:The Pancreas tissue lysate of Rat (43ug))

Related Product Information for anti-RHOBTB3 antibody

The Rho subfamily of Ras-related GTPases controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling and cell growth. RHOBTB1 (Rho-related BTB domain-containing protein 1) and RHOBTB3 (Rho-related BTB domain-containing protein 3) each contain two BTB (POZ) domains and belong to the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. While both RHOBTB1 and RHOBTB3 are expressed ubiquitously, RHOBTB1 is found at high levels in placenta, stomach, testis, kidney and skeletal muscle, whereas RHOBTB3 is found at high levels in neural and cardiac tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 115430108
• NCBI GeneID: 22836
• NCBI Accession #: NP_055714.3
• NCBI GenBank Nucleotide #: NP_055714.3
• UniProt Accession #: O94955
• Molecular Weight: ~ 69kDa
• NCBI Official Full Name: rho-related BTB domain-containing protein 3
• NCBI Official Synonym Full Names: Rho related BTB domain containing 3
• NCBI Official Symbol: RHOBTB3
• NCBI Protein Information: rho-related BTB domain-containing protein 3
• UniProt Protein Name: Rho-related BTB domain-containing protein 3
• Protein Family: Rho-related BTB domain-containing protein
• UniProt Gene Name: RHOBTB3
• UniProt Synonym Gene Names: KIAA0878
• UniProt Entry Name: RHBT3_HUMAN

NCBI Description

RHOBTB3 is a member of the evolutionarily conserved RHOBTB subfamily of Rho GTPases. For background information on RHOBTBs, see RHOBTB1 (MIM 607351).[supplied by OMIM, Apr 2004]

Uniprot Description

RHOBTB3: Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

Protein type: EC 3.6.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: Golgi apparatus

Molecular Function: protein binding; GTP binding; ATPase activity; ATP binding; Rab GTPase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi