Human Rho Related BTB Domain Containing Protein 3 (RHOBTB3) ELISA Kit | RHOBTB3 elisa kit

Human Rho Related BTB Domain Containing Protein 3 (RHOBTB3) ELISA Kit

Cat. Num. AAA2707778

• Reactivity: Human
• Synonyms: Rho Related BTB Domain Containing Protein 3 (RHOBTB3); Human Rho Related BTB Domain Containing Protein 3 (RHOBTB3) ELISA Kit; RHOBTB3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rho Related BTB Domain Containing Protein 3 (RHOBTB3).; No significant cross-reactivity or interference between Rho Related BTB Domain Containing Protein 3 (RHOBTB3) and analogues was observed.
• Sequence Length: 611

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.057ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rho Related BTB Domain Containing Protein 3 (RHOBTB3) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rho Related BTB Domain Containing Protein 3 (RHOBTB3) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RHOBTB3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho Related BTB Domain Containing Protein 3 (RHOBTB3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho Related BTB Domain Containing Protein 3 (RHOBTB3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho Related BTB Domain Containing Protein 3 (RHOBTB3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho Related BTB Domain Containing Protein 3 (RHOBTB3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115430108
• NCBI GeneID: 22836
• NCBI GenBank Nucleotide #: NP_055714.3
• UniProt Accession #: O94955
• NCBI Official Full Name: rho-related BTB domain-containing protein 3
• NCBI Official Synonym Full Names: Rho related BTB domain containing 3
• NCBI Official Symbol: RHOBTB3
• NCBI Protein Information: rho-related BTB domain-containing protein 3
• UniProt Protein Name: Rho-related BTB domain-containing protein 3
• Protein Family: Rho-related BTB domain-containing protein
• UniProt Gene Name: RHOBTB3
• UniProt Synonym Gene Names: KIAA0878
• UniProt Entry Name: RHBT3_HUMAN

NCBI Description

RHOBTB3 is a member of the evolutionarily conserved RHOBTB subfamily of Rho GTPases. For background information on RHOBTBs, see RHOBTB1 (MIM 607351).[supplied by OMIM, Apr 2004]

Uniprot Description

RHOBTB3: Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

Protein type: EC 3.6.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 5q15

Cellular Component: Golgi apparatus

Molecular Function: protein binding; GTP binding; ATPase activity; ATP binding; Rab GTPase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi