Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot (WB) analysis of RhoBTB1/2 (T85) pAb at 1:1000 dilutionLane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug))

Rabbit RhoBTB1/2 Polyclonal Antibody | anti-RhoBTB1/2 antibody

RhoBTB1/2 (T85) Polyclonal Antibody

Gene Names
RHOBTB2; p83; DBC2; EIEE64
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RhoBTB1/2; Polyclonal Antibody; RhoBTB1/2 (T85) Polyclonal Antibody; Rho-related BTB domain-containing protein 2; Deleted in breast cancer 2 gene protein; p83; RHOBTB2; DBC2; KIAA0717; Rho-related BTB domain-containing protein 1; RHOBTB1; KIAA0740; anti-RhoBTB1/2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RhoBTB1/2 (T85) polyclonal antibody detects endogenous levels of RhoBTB1/2 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
727
Applicable Applications for anti-RhoBTB1/2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 52-98 of Human RhoBTB1.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RhoBTB1/2 (T85) pAb at 1:1000 dilutionLane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug))

Western Blot (WB) (Western Blot (WB) analysis of RhoBTB1/2 (T85) pAb at 1:1000 dilutionLane1:The Kidney tissue lysate of Mouse (40ug) Lane2:The Kidney tissue lysate of Rat (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of RhoBTB1/2 (T85) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)

Immunohistochemistry (IHC) (Immunohistochemistry (IHC) analyzes of RhoBTB1/2 (T85) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.)
Related Product Information for anti-RhoBTB1/2 antibody
RHOBTB1 (Rho-related BTB domain-containing protein 1) and RHOBTB3 (Rho-related BTB domain-containing protein 3) each contain two BTB (POZ) domains and belong to the RhoBTB subfamily of Rho GTPases. Members of the RhoBTB subfamily are evolutionarily conserved and are characterized by a proline-rich region, a GTPase domain and two tandem BTB repeats. While both RHOBTB1 and RHOBTB3 are expressed ubiquitously, RHOBTB1 is found at high levels in placenta, stomach, testis, kidney and skeletal muscle, whereas RHOBTB3 is found at high levels in neural and cardiac tissues. RHOBTB1 is thought to play a role in GTPase-mediated signaling and may participate in organization of the Actin filament system. Additionally, RHOBTB1 expression is decreased in head and neck carcinomas, suggesting a possible role for RHOBTB1 as a tumor suppressor.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
~ 83kDa
NCBI Official Full Name
DBC2
NCBI Official Synonym Full Names
Rho related BTB domain containing 2
NCBI Official Symbol
RHOBTB2
NCBI Official Synonym Symbols
p83; DBC2; EIEE64
NCBI Protein Information
rho-related BTB domain-containing protein 2
UniProt Protein Name
Rho-related BTB domain-containing protein 2
UniProt Gene Name
RHOBTB2
UniProt Synonym Gene Names
DBC2; KIAA0717
UniProt Entry Name
RHBT2_HUMAN

NCBI Description

The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

RHOBTB2: is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Protein type: G protein; G protein, monomeric, Rho; G protein, monomeric

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: plasma membrane; cytosol

Molecular Function: GTP binding

Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction

Research Articles on RhoBTB1/2

Similar Products

Product Notes

The RhoBTB1/2 rhobtb2 (Catalog #AAA3002702) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RhoBTB1/2 (T85) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RhoBTB1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the RhoBTB1/2 rhobtb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RhoBTB1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.