Rabbit anti-Human RCN1 Polyclonal Antibody | anti-RCN1 antibody

RCN1, CT (RCN1, RCN, Reticulocalbin-1) (FITC)

Cat. Num. AAA6341062

• Gene Names: RCN1; RCN; RCAL; PIG20; HEL-S-84
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RCN1; Polyclonal Antibody; CT (RCN1; RCN; Reticulocalbin-1) (FITC); Reticulocalbin-1; anti-RCN1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RCN1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-331 amino acids from the C-terminal region of human RCN1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RCN1 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the RCN1 antibody detected the RCN1 protein (arrow).)

Related Product Information for anti-RCN1 antibody

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.

Product Categories/Family for anti-RCN1 antibody

Antibodies; Proteins

References

Favor, J., et al. Genetics 182(4):1077-1088(2009). Hansen, G.A., et al. Mol. Cell. Biochem. 320 (1-2), 25-33 (2009): Cooper, C.R., et al. J. Cell. Biochem. 104(6):2298-2309(2008). Lim, J., et al. Cell 125(4):801-814(2006). Taylor, T.D., et al. Nature 440(7083):497-500(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 4506455
• NCBI GeneID: 5954
• NCBI Accession #: NP_002892.1
• NCBI GenBank Nucleotide #: NM_002901.2
• UniProt Accession #: Q15293; B7Z1M1; D3DR00
• Molecular Weight: 33,026 Da
• NCBI Official Full Name: reticulocalbin-1
• NCBI Official Synonym Full Names: reticulocalbin 1, EF-hand calcium binding domain
• NCBI Official Symbol: RCN1
• NCBI Official Synonym Symbols: RCN; RCAL; PIG20; HEL-S-84
• NCBI Protein Information: reticulocalbin-1; epididymis secretory protein Li 84; proliferation-inducing gene 20
• UniProt Protein Name: Reticulocalbin-1
• Protein Family: Reticulocalbin
• UniProt Gene Name: RCN1
• UniProt Synonym Gene Names: RCN
• UniProt Entry Name: RCN1_HUMAN

NCBI Description

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding. In human endothelial and prostate cancer cell lines this protein localizes to the plasma membrane.[provided by RefSeq, Jan 2009]

Uniprot Description

RCN1: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen

Molecular Function: protein binding; calcium ion binding

Biological Process: camera-type eye development; in utero embryonic development