Rabbit Rheb Polyclonal Antibody | anti-RHEB antibody

Rheb Antibody

Cat. Num. AAA150665

• Gene Names: RHEB; RHEB2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Rheb Antibody is affinity chromatography purified via peptide column.
• Synonyms: Rheb; Polyclonal Antibody; Rheb Antibody; RHEB2; GTP-binding protein Rheb; Ras homolog enriched in brain; anti-RHEB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Rheb Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 184

• Applicable Applications for anti-RHEB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Rheb antibody can be used for the detection of Rheb by Western blot at 1 to 4 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Rheb antibody was raised against a 15 amino acid synthetic peptide from the middle region of human Rheb.
• Buffer: Rheb Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Rheb antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Rheb in mouse brain cell lysate with Rheb antibody at (A) 1, (B) 2, and (C) 4 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of Rheb in mouse brain tissue with Rheb antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of Rheb in Mouse Brain cells with Rheb antibody at 20 μg/mL.)

Related Product Information for anti-RHEB antibody

Rheb Antibody: Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demonstrate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

NCBI and Uniprot Product Information

• NCBI GI #: 16740561
• NCBI GeneID: 6009
• UniProt Accession #: Q15382; Q53Y56; Q99444; B3KWN6; D3DX13
• Molecular Weight: 19; 20 kDa
• NCBI Official Full Name: Ras homolog enriched in brain
• NCBI Official Synonym Full Names: Ras homolog enriched in brain
• NCBI Official Symbol: RHEB
• NCBI Official Synonym Symbols: RHEB2
• NCBI Protein Information: GTP-binding protein Rheb; Ras homolog enriched in brain 2
• UniProt Protein Name: GTP-binding protein Rheb
• Protein Family: Rheb-like protein
• UniProt Gene Name: RHEB
• UniProt Synonym Gene Names: RHEB2
• UniProt Entry Name: RHEB_HUMAN

NCBI Description

This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]

Uniprot Description

RHEB: Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. Binds to mTORC1 in a guanyl nucleotide-independent manner. Interacts directly with MTOR, MLST8 and RPTOR. Interacts with TSC2. Ubiquitous. Highest levels observed in skeletal and cardiac muscle. Alternates between an inactive form bound to GDP and an active form bound to GTP. Inactivated by TSC1-TSC2 via the GTPase activating protein (GAP) domain of TSC2. Belongs to the small GTPase superfamily. Rheb family.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rheb

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; spliceosome; endoplasmic reticulum membrane; membrane; cell soma; dendrite; plasma membrane; cytosol

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; metal ion binding; protein kinase binding

Biological Process: metabolic process; small GTPase mediated signal transduction; insulin receptor signaling pathway; signal transduction; cell cycle arrest; positive regulation of TOR signaling pathway