Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit | AVPR1B elisa kit

Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit

Cat. Num. AAA2703092

• Gene Names: AVPR1B; V1bR; AVPR3
• Reactivity: Mouse
• Synonyms: Arginine Vasopressin Receptor 1B (AVPR1B); Mouse Arginine Vasopressin Receptor 1B (AVPR1B) ELISA Kit; AVPR1-B; AVPR3; Vasopressin 3 Receptor; Antidiuretic Hormone Receptor 1b; AVPR1B elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arginine Vasopressin Receptor 1B (AVPR1B).; No significant cross-reactivity or interference between Arginine Vasopressin Receptor 1B (AVPR1B) and analogues was observed.
• Sequence Length: 424

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arginine Vasopressin Receptor 1B (AVPR1B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arginine Vasopressin Receptor 1B (AVPR1B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AVPR1B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arginine Vasopressin Receptor 1B (AVPR1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arginine Vasopressin Receptor 1B (AVPR1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arginine Vasopressin Receptor 1B (AVPR1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arginine Vasopressin Receptor 1B (AVPR1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74418840
• NCBI GeneID: 553
• UniProt Accession #: P47901; Q5TZ00; B0M0J6
• Molecular Weight: 46,971 Da
• NCBI Official Full Name: arginine vasopressin receptor 1B
• NCBI Official Synonym Full Names: arginine vasopressin receptor 1B
• NCBI Official Symbol: AVPR1B
• NCBI Official Synonym Symbols: V1bR; AVPR3
• NCBI Protein Information: vasopressin V1b receptor
• UniProt Protein Name: Vasopressin V1b receptor
• Protein Family: Vasopressin V1b receptor
• UniProt Gene Name: AVPR1B
• UniProt Synonym Gene Names: AVPR3; VPR3; V1bR

NCBI Description

The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1A, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor is primarily located in the anterior pituitary, where it stimulates ACTH release. It is expressed at high levels in ACTH-secreting pituitary adenomas as well as in bronchial carcinoids responsible for the ectopic ACTH syndrome. A spliced antisense transcript of this gene has been reported but its function is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system.