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Western Blot (WB) (Western Blot: Sample: Rat Lymph node lysate; Primary Ab: 1ug/ml Rabbit Anti-Human LMAN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Human Lectin Polyclonal Antibody | anti-LMAN2 antibody

Polyclonal Antibody to Lectin, Mannose Binding 2 (LMAN2)

Gene Names
LMAN2; GP36B; VIP36; C5orf8
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Lectin; Polyclonal Antibody; Polyclonal Antibody to Lectin; Mannose Binding 2 (LMAN2); anti-LMAN2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LMAN2. It has beenselected for its ability to recognize LMAN2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence Length
169
Applicable Applications for anti-LMAN2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
LMAN2 (Asp45~Arg322)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Rat
Conjugate
No Conjugate
Immunogen
Recombinant LMAN2 (Asp45~Arg322) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2080238
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Rat Lymph node lysate; Primary Ab: 1ug/ml Rabbit Anti-Human LMAN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Rat Lymph node lysate; Primary Ab: 1ug/ml Rabbit Anti-Human LMAN2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Human Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Human Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,229 Da
NCBI Official Full Name
vesicular integral-membrane protein VIP36
NCBI Official Synonym Full Names
lectin, mannose binding 2
NCBI Official Symbol
LMAN2
NCBI Official Synonym Symbols
GP36B; VIP36; C5orf8
NCBI Protein Information
vesicular integral-membrane protein VIP36
UniProt Protein Name
Vesicular integral-membrane protein VIP36
Protein Family
UniProt Gene Name
LMAN2
UniProt Synonym Gene Names
C5orf8; VIP36

NCBI Description

This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking and quality control. [provided by RefSeq, Oct 2008]

Uniprot Description

LMAN2: Plays a role as an intracellular lectin in the early secretory pathway. Interacts with N-acetyl-D-galactosamine and high-mannose type glycans and may also bind to O-linked glycans. Involved in the transport and sorting of glycoproteins carrying high mannose-type glycans.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: cell surface; endoplasmic reticulum membrane; endoplasmic reticulum-Golgi intermediate compartment; endoplasmic reticulum-Golgi intermediate compartment membrane; extracellular space; Golgi apparatus; Golgi membrane; integral component of plasma membrane

Molecular Function: carbohydrate binding; heat shock protein binding; mannose binding; metal ion binding; protein binding

Biological Process: positive regulation of phagocytosis; protein transport; retrograde vesicle-mediated transport, Golgi to ER

Research Articles on LMAN2

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Product Notes

The LMAN2 lman2 (Catalog #AAA2027692) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lectin, Mannose Binding 2 (LMAN2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lectin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the LMAN2 lman2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lectin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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