Western Blot (WB)
(Western Blot (WB) analysis of RHD polyclonal antibody at 1:500 dilutionLane1:The Brain tissue lysate of Rat (30ug) Lane2:The Brain tissue lysate of Mouse (30ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))
Rabbit RHD Polyclonal Antibody | anti-RHD antibody
RHD Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RHD; Polyclonal Antibody; RHD Polyclonal Antibody; Blood group Rh(D) polypeptide; RHXIII; Rh polypeptide 2; RhPII; Rhesus D antigen; CD240D; anti-RHD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RHD polyclonal antibody detects endogenous levels of RHD protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
321
Applicable Applications for anti-RHD antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
Synthetic peptide, corresponding to amino acids 168-213 of Human RHD.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of RHD polyclonal antibody at 1:500 dilutionLane1:The Brain tissue lysate of Rat (30ug) Lane2:The Brain tissue lysate of Mouse (30ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of RHD polyclonal antibody at 1:500 dilutionLane1:The Brain tissue lysate of Rat (30ug) Lane2:The Brain tissue lysate of Mouse (30ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug))
Related Product Information for anti-RHD antibody
The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans (1). Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1-4). These tightly linked genes map to human chromosomal position 1p34.1-1p36 (2, 3). The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2, 5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1, 2, 6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2, 4, 5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity (2). The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50) (2).
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 45kDa
NCBI Official Full Name
blood group Rh(D) polypeptide isoform 2
UniProt Protein Name
Blood group Rh(D) polypeptide
UniProt Gene Name
RHD
UniProt Synonym Gene Names
RhPII
Uniprot Description
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane.
Similar Products
Product Notes
The RHD rhd (Catalog #AAA3007336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHD Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the RHD rhd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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