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Western Blot (WB) (Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody. Lane 1: MAPK9 transfected lysate (48.1kD). Lane 2: Non-transfected lysate.)

Mouse anti-Human MAPK9 Monoclonal Antibody | anti-MAPK9 antibody

MAPK9 (Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, JNK-55, Stress-activated Protein Kinase 1a, SAPK1a, Stress-activated Protein Kinase JNK2, c-Jun N-terminal Kinase 2, JNK2, PRKM9, SAPK1A) (PE)

Gene Names
MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MAPK9; Monoclonal Antibody; MAPK9 (Mitogen-activated Protein Kinase 9; MAP Kinase 9; MAPK 9; JNK-55; Stress-activated Protein Kinase 1a; SAPK1a; Stress-activated Protein Kinase JNK2; c-Jun N-terminal Kinase 2; JNK2; PRKM9; SAPK1A) (PE); anti-MAPK9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1C1-3A8
Specificity
Recognizes human MAPK9.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MAPK9 antibody
ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 3ug/ml
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to human MAPK9, aa1-425 (AAH32539) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody. Lane 1: MAPK9 transfected lysate (48.1kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody. Lane 1: MAPK9 transfected lysate (48.1kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3ug/ml])

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged MAPK9 is ~3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged MAPK9 is ~3ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Product Categories/Family for anti-MAPK9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
27,334 Da
NCBI Official Full Name
Homo sapiens mitogen-activated protein kinase 9, mRNA
NCBI Official Synonym Full Names
mitogen-activated protein kinase 9
NCBI Official Symbol
MAPK9
NCBI Official Synonym Symbols
JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
NCBI Protein Information
mitogen-activated protein kinase 9

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]

Research Articles on MAPK9

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Product Notes

The MAPK9 (Catalog #AAA6158729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAPK9 (Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, JNK-55, Stress-activated Protein Kinase 1a, SAPK1a, Stress-activated Protein Kinase JNK2, c-Jun N-terminal Kinase 2, JNK2, PRKM9, SAPK1A) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 3ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAPK9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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