Western Blot (WB)
(Western blot analysis of RHBG Antibody (C-term) in K562 cell line lysates (35ug/lane). RHBG (arrow) was detected using the purified Pab.)
Rabbit anti-Human RHBG Polyclonal Antibody | anti-RHBG antibody
RHBG, CT (RHBG, Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein) (FITC)
Gene Names
RHBG; SLC42A2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RHBG; Polyclonal Antibody; CT (RHBG; Ammonium transporter Rh type B; Rhesus blood group family type B glycoprotein) (FITC); Rhesus blood group family type B glycoprotein; anti-RHBG antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-RHBG antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RHBG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 421~450 amino acids from the C-terminal region of human RHBG.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of RHBG Antibody (C-term) in K562 cell line lysates (35ug/lane). RHBG (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of RHBG Antibody (C-term) in K562 cell line lysates (35ug/lane). RHBG (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(RHBG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RHBG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RHBG Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RHBG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RHBG Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RHBG Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RHBG antibody
RHBG encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N-and C-termini.
Product Categories/Family for anti-RHBG antibody
References
Han, K.H., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 297 (1), L153-L163 (2009). Brown, A.C., et al. Am. J. Physiol. Renal Physiol. 296 (6), F1279-F1290 (2009). Sohet, F., et al. J. Biol. Chem. 283(39):26557-26567(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,092 Da
NCBI Official Full Name
ammonium transporter Rh type B isoform a
NCBI Official Synonym Full Names
Rh family, B glycoprotein (gene/pseudogene)
NCBI Official Symbol
RHBG
NCBI Official Synonym Symbols
SLC42A2
NCBI Protein Information
ammonium transporter Rh type B; Rhesus blood group, B glycoprotein
UniProt Protein Name
Ammonium transporter Rh type B
Protein Family
UniProt Gene Name
RHBG
UniProt Synonym Gene Names
Rh family type B glycoprotein; Rh type B glycoprotein
UniProt Entry Name
RHBG_HUMAN
NCBI Description
This gene encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
Functions as a specific ammonium transporter.
Research Articles on RHBG
Similar Products
Product Notes
The RHBG rhbg (Catalog #AAA6341843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RHBG, CT (RHBG, Ammonium transporter Rh type B, Rhesus blood group family type B glycoprotein) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RHBG can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RHBG rhbg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHBG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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