Rabbit anti-Human GABRB1 Polyclonal Antibody | anti-GABRB1 antibody

GABRB1 (Gamma-aminobutyric Acid Receptor Subunit beta-1, GABA(A) Receptor Subunit beta-1)

Cat. Num. AAA6013238

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: GABRB1; Polyclonal Antibody; GABRB1 (Gamma-aminobutyric Acid Receptor Subunit beta-1; GABA(A) Receptor Subunit beta-1); Anti -GABRB1 (Gamma-aminobutyric Acid Receptor Subunit beta-1; anti-GABRB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human GABRB1. Species sequence homology: mouse (89%), rat (89%), bovine (89%) and zebrafish (90%).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7, 0.01% thimerosal, 10% glycerol.

• Applicable Applications for anti-GABRB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:500-1:3000; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Recombinant corresponding to aa1-226 from human GABA A Receptor beta 1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Sample (30ug whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: Hep G2. F: MOLT4. G: Raji. 10% SDS PAGE. GABRB1 antibody diluted at 1:1000)

Related Product Information for anti-GABRB1 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features. GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain.

Product Categories/Family for anti-GABRB1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 194097327
• NCBI GeneID: 2560
• NCBI Accession #: NP_000803.2
• NCBI GenBank Nucleotide #: NM_000812.3
• UniProt Accession #: P18505; Q16166; Q8TBK3; B2R6U7
• Molecular Weight: 54,235 Da
• NCBI Official Full Name: gamma-aminobutyric acid receptor subunit beta-1
• NCBI Official Synonym Full Names: gamma-aminobutyric acid (GABA) A receptor, beta 1
• NCBI Official Symbol: GABRB1
• NCBI Protein Information: gamma-aminobutyric acid receptor subunit beta-1; GABA(A) receptor, beta 1; GABA(A) receptor subunit beta-1
• UniProt Protein Name: Gamma-aminobutyric acid receptor subunit beta-1
• Protein Family: Gamma-aminobutyric acid receptor
• UniProt Gene Name: GABRB1
• UniProt Entry Name: GBRB1_HUMAN

NCBI Description

The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. This gene encodes GABA A receptor, beta 1 subunit. It is mapped to chromosome 4p12 in a cluster comprised of genes encoding alpha 4, alpha 2 and gamma 1 subunits of the GABA A receptor. Alteration of this gene is implicated in the pathogenetics of schizophrenia. [provided by RefSeq, Jul 2008]

Uniprot Description

GABRB1: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB1 sub-subfamily.

Protein type: Membrane protein, multi-pass; Channel, chloride; Channel, ligand-gated; Membrane protein, integral; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: postsynaptic membrane; integral to plasma membrane; plasma membrane; cell junction

Molecular Function: chloride channel activity; GABA-A receptor activity; ligand-gated ion channel activity; extracellular ligand-gated ion channel activity

Biological Process: synaptic transmission; transport; ion transport; signal transduction; transmembrane transport