Human Complement C5 ELISA Kit | C5 elisa kit

Human Complement C5 ELISA Kit

Cat. Num. AAA764102

• Gene Names: C5; C5D; C5a; C5b; ECLZB; CPAMD4
• Reactivity: Human
• Synonyms: Complement C5; Human Complement C5 ELISA Kit; C5 (Complement Component 5); C5 elisa kit

• Reactivity: Human
• Sequence Length: 1676

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for C5 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-C5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-C5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38016947
• NCBI GeneID: 727
• NCBI Accession #: NP_001726.2
• NCBI GenBank Nucleotide #: NM_001735.2
• Molecular Weight: 188,305 Da
• NCBI Official Full Name: complement C5 isoform 1 preproprotein
• NCBI Official Synonym Full Names: complement component 5
• NCBI Official Symbol: C5
• NCBI Official Synonym Symbols: C5D; C5a; C5b; ECLZB; CPAMD4
• NCBI Protein Information: complement C5
• UniProt Protein Name: Complement C5
• Protein Family: Complement C5
• UniProt Gene Name: C5
• UniProt Synonym Gene Names: CPAMD4
• UniProt Entry Name: CO5_HUMAN

NCBI Description

This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]

Uniprot Description

C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9q33-q34

Cellular Component: extracellular region; extracellular space; membrane attack complex

Molecular Function: chemokine activity; endopeptidase inhibitor activity; protein binding; receptor binding

Biological Process: activation of MAPK activity; cell surface receptor linked signal transduction; chemotaxis; complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis; G-protein coupled receptor protein signaling pathway; in utero embryonic development; inflammatory response; innate immune response; positive regulation of angiogenesis; regulation of complement activation; response to stress

Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To