Rabbit CDC37 Polyclonal Antibody | anti-CDC37 antibody

CDC37 Antibody

Cat. Num. AAA9602004

• Gene Names: CDC37; P50CDC37
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: CDC37; Polyclonal Antibody; CDC37 Antibody; CDC 37; Cdc37; CDC37 cell division cycle 37 homolog; CDC37 cell division cycle 37 S cerevisiae homolog; CDC37 cell division cycle 37; S cerevisiae; homolog of; Cdc37 homolog; CDC37 protein; CDC37_HUMAN; CDC37A; cell division cycle 37; Cell division cycle 37 homolog; Hsp90 chaperone protein kinase targeting subunit; Hsp90 chaperone protein kinase targeting subunit p50Cdc37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co chaperone Cdc37; Hsp90 co-chaperone Cdc37; p50; p50Cdc37; S cerevisiae hypothetical protein CDC37; anti-CDC37 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CDC37 antibody detects endogenous levels of total CDC37
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 378

• Applicable Applications for anti-CDC37 antibody: Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human CDC37
• Subcellular Location: Cytoplasm.
• Predicted Cross Reactivity: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9602004 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Immunohistochemistry (IHC)

(MBS9602004 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of CDC37 expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-CDC37 antibody

Description: The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction.
Function: Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206).
Subunit Structure: Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2 (PubMed:29127155). Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23 (PubMed:29127155). Forms a complex with Hsp90/HSP90AB1 and CDK6 (PubMed:9482106). Interacts with HSP90AA1 (PubMed:23569206, PubMed:27353360). Interacts with AR, CDK4, CDK6 and EIF2AK1 (PubMed:11036079, PubMed:11085988, PubMed:9150368, PubMed:9482106). Interacts with RB1 (By similarity). Interacts with KSR1 (PubMed:10409742). Interacts with FLCN, FNIP1 and FNIP2 (PubMed:27353360).
Post-translational Modifications: Constitutively sumoylated by UBE2I.
Similarity: Belongs to the CDC37 family.

NCBI and Uniprot Product Information

• NCBI GI #: 5901922
• NCBI GeneID: 11140
• NCBI Accession #: NP_008996.1
• NCBI GenBank Nucleotide #: NM_007065.3
• UniProt Accession #: Q16543; Q53YA2
• Molecular Weight: Observed: 44 kDa; Predicted: 45 kDa
• NCBI Official Full Name: hsp90 co-chaperone Cdc37
• NCBI Official Synonym Full Names: cell division cycle 37
• NCBI Official Symbol: CDC37
• NCBI Official Synonym Symbols: P50CDC37
• NCBI Protein Information: hsp90 co-chaperone Cdc37
• UniProt Protein Name: Hsp90 co-chaperone Cdc37
• Protein Family: Hsp90 co-chaperone
• UniProt Gene Name: CDC37
• UniProt Synonym Gene Names: CDC37A

NCBI Description

The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008]

Uniprot Description

Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206).