Human CD56/NCAM-1 ELISA Kit | NCAM1 elisa kit

Human CD56/NCAM-1 PicoKine ELISA Kit

Cat. Num. AAA175916

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Human
• Synonyms: CD56/NCAM-1; Human CD56/NCAM-1 PicoKine ELISA Kit; Neural cell adhesion molecule 1; neural cell adhesion molecule 1; CD56; N-CAM-1; NCAM-1; NCAM1; NCAM1_HUMAN; NCAM1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human MSTN
• Sequence Length: 858

• Samples: Cell Culture Supernates, Tissue, Serum And Plasma(Heparin Or Edta)
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NCAM1 elisa kit

Principle of the Assay: The Picokine Human MSTN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human MSTN with a 96-well strip plate that is pre-coated with antibody specific for MSTN. The detection antibody is a biotinylated antibody specific for MSTN. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human MSTN with immunogen: Expression system for standard: NSO, Immunogen sequence: D267-S375. The kit is analytically validated with ready to use reagents. To measure Human MSTN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human MSTN in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human MSTN in the sample!!Background/Introduction: Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 2q32.2. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

NCBI and Uniprot Product Information

• NCBI GI #: 205830665
• NCBI GeneID: 4684
• UniProt Accession #: P13591; P13592; P13593; Q05C58; Q15829; Q16180; Q16209; Q59FL7; Q86X47; Q96CJ3; A8K8T8
• Molecular Weight: 40,760 Da
• NCBI Official Full Name: Neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: Golgi membrane; cell surface; membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: identical protein binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion