Western Blot (WB)
(Western blot analysis of RFC3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). RFC3 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse RFC3 Polyclonal Antibody | anti-RFC3 antibody
RFC3, CT (RFC3, Replication factor C subunit 3, Activator 1 38kD subunit, Activator 1 subunit 3, Replication factor C 38kD subunit) (PE)
ELISA: 1:1,000
WB: 1:50-100
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western blot analysis of RFC3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). RFC3 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of RFC3 Antibody (C-term) in mouse kidney tissue lysates (35ug/lane). RFC3 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(RFC3 Antibody (C-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RFC3 Antibody (C-term) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
RFC3: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. Belongs to the activator 1 small subunits family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 13q13.2
Cellular Component: nucleoplasm; DNA replication factor C complex; nucleus
Molecular Function: protein binding; DNA binding; ATPase activity; DNA clamp loader activity
Biological Process: telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; response to organophosphorus; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; DNA repair; DNA replication; telomere maintenance
Research Articles on RFC3
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Product Notes
The RFC3 rfc3 (Catalog #AAA6341465) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RFC3, CT (RFC3, Replication factor C subunit 3, Activator 1 38kD subunit, Activator 1 subunit 3, Replication factor C 38kD subunit) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RFC3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RFC3 rfc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RFC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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