Rabbit anti-Human, Mouse GRK1 Polyclonal Antibody | anti-GRK1 antibody

GRK1, CT (G Protein-coupled Receptor Kinase 1, GPRK1, Rhodopsin Kinase, RHOK, RK)

Cat. Num. AAA623874

• Gene Names: GRK1; RK; RHOK; GPRK1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: GRK1; Polyclonal Antibody; CT (G Protein-coupled Receptor Kinase 1; GPRK1; Rhodopsin Kinase; RHOK; RK); Anti -GRK1; anti-GRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GRK1. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-GRK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the C-terminal region of human GRK1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GRK1 Pab is used in Western blot to detect GRK1 in HeLa cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK1 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-GRK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK) The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-GRK1 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 223461377
• NCBI GeneID: 6011
• UniProt Accession #: Q15835; Q53X14; O95098; Q6VTF2; Q71VB6
• Molecular Weight: 63,526 Da
• NCBI Official Full Name: G protein-coupled receptor kinase 1
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 1
• NCBI Official Symbol: GRK1
• NCBI Official Synonym Symbols: RK; RHOK; GPRK1
• NCBI Protein Information: rhodopsin kinase
• UniProt Protein Name: Rhodopsin kinase
• UniProt Gene Name: GRK1
• UniProt Synonym Gene Names: RHOK
• UniProt Entry Name: RK_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq]

Uniprot Description

GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.14; Kinase, protein; Protein kinase, AGC; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 13q34

Molecular Function: G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity

Biological Process: rhodopsin mediated signaling; phototransduction, visible light; post-embryonic retina morphogenesis in camera-type eye; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis

Disease: Oguchi Disease 2