Rabbit anti-Human REEP2 Polyclonal Antibody | anti-REEP2 antibody

Anti-REEP2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108614

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: REEP2; Polyclonal Antibody; Anti-REEP2 Antibody; Rabbit Polyclonal; REEP2 Antibody; Rabbit PAb; Antigen Affinity Purified; receptor accessory protein 2; Anti-C5orf19 Antibody; Anti-SGC32445 Antibody; Anti-SPG72 Antibody; anti-REEP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human REEP2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-REEP2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human REEP2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human REEP2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-REEP2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:28 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human REEP2 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(REEP2 was immunoprecipitated using: Lane A:0.5 mg U-251 MG Whole Cell Lysate 4 uL anti-REEP2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-REEP2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa Observed band size :26 kDa)

Related Product Information for anti-REEP2 antibody

Mutations in the REEP1 gene that encodes an endoplasmic reticulum-shaping protein are well-known causes of SPG31, a common autosomal dominant spastic paraplegia. A closely-related gene, REEP2, is mutated in SPG72, with both autosomal and recessive inheritances. REEP2 belongs to a family of proteins that shape the endoplasmic reticulum, an organelle that was altered in fibroblasts from an affected subject. In vitro, the p.Val36Glu variant in the autosomal-dominant family had a dominant-negative effect; it inhibited the normal binding of wild-type REEP2 to membranes. The missense substitution p.Phe72Tyr, in the recessive family, decreased the affinity of the mutant protein for membranes that, together with the splice site mutation, is expected to cause complete loss of REEP2 function.

References

Roda RH, et al. (2017) De novo reep2 missense mutation in pure hereditary spastic paraplegia. Ann Clin Transl Neurol 4 (5): 347-350.Esteves T, et al. (2014) Loss of association of reep2 with membranes leads to hereditary spastic paraplegia. Am J Hum Genet 94 (2): 268-277.