Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Rabbit anti-Human RDH16 Polyclonal Antibody | anti-RDH16 antibody
RDH16, NT (RDH16, RODH4, Retinol dehydrogenase 16, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Sterol/retinol dehydrogenase) (APC)
Gene Names
RDH16; RODH-4; SDR9C8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
RDH16; Polyclonal Antibody; NT (RDH16; RODH4; Retinol dehydrogenase 16; Microsomal NAD(+)-dependent retinol dehydrogenase 4; Sterol/retinol dehydrogenase) (APC); Sterol/retinol dehydrogenase; anti-RDH16 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-RDH16 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
RDH16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human RDH16.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Immunohistochemistry (IHC)
(RDH16 Antibody (N-term immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH16 Antibody (N-term for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RDH16 Antibody (N-term immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH16 Antibody (N-term for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RDH16 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RDH16 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RDH16 antibody
Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.
Product Categories/Family for anti-RDH16 antibody
References
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009): Lapshina, E.A., et al. Biochemistry 42(3):776-784(2003). Cain, J.M., et al. Am. J. Obstet. Gynecol. 186(4):675-683(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,673 Da
NCBI Official Full Name
retinol dehydrogenase 16
NCBI Official Synonym Full Names
retinol dehydrogenase 16 (all-trans)
NCBI Official Symbol
RDH16
NCBI Official Synonym Symbols
RODH-4; SDR9C8
NCBI Protein Information
retinol dehydrogenase 16; sterol/retinol dehydrogenase; retinol dehydrogenase 16 (all-trans and 13-cis); microsomal NAD+-dependent retinol dehydrogenase 4; microsomal NAD(+)-dependent retinol dehydrogenase 4; short chain dehydrogenase/reductase family 9C,
UniProt Protein Name
Retinol dehydrogenase 16
Protein Family
UniProt Gene Name
RDH16
UniProt Synonym Gene Names
RODH4; RoDH-4
UniProt Entry Name
RDH16_HUMAN
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Product Notes
The RDH16 rdh16 (Catalog #AAA6341148) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RDH16, NT (RDH16, RODH4, Retinol dehydrogenase 16, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Sterol/retinol dehydrogenase) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RDH16 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the RDH16 rdh16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RDH16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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