Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Rabbit anti-Human RDH16 Polyclonal Antibody | anti-RDH16 antibody
RDH16, NT (RDH16, RODH4, Retinol dehydrogenase 16, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Sterol/retinol dehydrogenase)
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
RDH16; Polyclonal Antibody; NT (RDH16; RODH4; Retinol dehydrogenase 16; Microsomal NAD(+)-dependent retinol dehydrogenase 4; Sterol/retinol dehydrogenase); Anti -RDH16; anti-RDH16 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-RDH16 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50
Immunogen
RDH16 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human RDH16.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Western Blot (WB)
(RDH16 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RDH16 antibody detected the RDH16 protein (arrow).)
Immunohistochemistry (IHC)
(RDH16 Antibody (N-term immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH16 Antibody (N-term for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(RDH16 Antibody (N-term immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH16 Antibody (N-term for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(RDH16 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(RDH16 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RDH16 antibody
Retinol dehydrogenase 16 (RDH16) is an oxidoreductase with a preference for NAD. It oxidizes all-trans-retinol and 13-cis-retinol to the corresponding aldehydes. RDH16 has higher activity towards CRBP-bound retinol than with free retinol. It oxidizes 3-alpha-hydroxysteroids and also oxidizes androstanediol and androsterone to dihydrotestosterone and androstanedione. RDH16 can also catalyze the reverse reaction.
Product Categories/Family for anti-RDH16 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
retinol dehydrogenase 16
NCBI Official Synonym Full Names
retinol dehydrogenase 16 (all-trans)<
NCBI Official Symbol
RDH16
NCBI Protein Information
retinol dehydrogenase 16; microsomal NAD+-dependent retinol dehydrogenase 4
Protein Family
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Product Notes
The RDH16 (Catalog #AAA646514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RDH16, NT (RDH16, RODH4, Retinol dehydrogenase 16, Microsomal NAD(+)-dependent retinol dehydrogenase 4, Sterol/retinol dehydrogenase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RDH16 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:50-100 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the RDH16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RDH16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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