Rabbit anti-Human RDH10 Polyclonal Antibody | anti-RDH10 antibody

RDH10, ID (RDH10, Retinol dehydrogenase 10) (APC)

Cat. Num. AAA6341126

• Gene Names: RDH10; SDR16C4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RDH10; Polyclonal Antibody; ID (RDH10; Retinol dehydrogenase 10) (APC); Retinol dehydrogenase 10; anti-RDH10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-RDH10 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113~143 amino acids from the Central region of human RDH10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(RDH10 Antibody (Center) western blot analysis in WiDr(lane 1),NCI-H460(lane 2),HepG2(lane 3) cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).)

Immunohistochemistry (IHC)

(RDH10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RDH10 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RDH10 antibody

RDH10 generates all-trans retinal from all-trans retinol and may plan an important role in the photic visual cycle. All-trans retinal is isomerized to 11-cis retinal by the retinal G protein-coupled receptor (RGR; MIM 600342) when the retinal pigment epithelium (RPE) is illuminated.

Product Categories/Family for anti-RDH10 antibody

Antibodies; Enzymes; Dehydrogenase

References

Bankovic, J., et al. Lung Cancer 67(2):151-159(2010). Takahashi, Y., et al. Biochem. J. 419(1):113-122(2009). Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 25282469
• NCBI GeneID: 157506
• NCBI Accession #: NP_742034.1
• NCBI GenBank Nucleotide #: NM_172037.4
• UniProt Accession #: Q8IZV5
• Molecular Weight: 38,087 Da
• NCBI Official Full Name: retinol dehydrogenase 10
• NCBI Official Synonym Full Names: retinol dehydrogenase 10 (all-trans)
• NCBI Official Symbol: RDH10
• NCBI Official Synonym Symbols: SDR16C4
• NCBI Protein Information: retinol dehydrogenase 10; short chain dehydrogenase/reductase family 16C, member 4
• UniProt Protein Name: Retinol dehydrogenase 10
• Protein Family: Retinol dehydrogenase
• UniProt Gene Name: RDH10
• UniProt Entry Name: RDH10_HUMAN

NCBI Description

This gene encodes a retinol dehydrogenase, which converts all-trans-retinol to all-trans-retinal, with preference for NADP as a cofactor. Studies in mice suggest that this protein is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. [provided by RefSeq, Dec 2011]

Uniprot Description

Function: Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol. Ref.1

Catalytic activity: All-trans-retinol + NADP+ = all-trans-retinal + NADPH. Ref.1

Pathway: Cofactor metabolism; retinol metabolism.

Subcellular location: Microsome membrane; Single-pass membrane protein

Potential. Endoplasmic reticulum membrane; Single-pass membrane protein

Potential Ref.1.

Tissue specificity: Detected in retina, kidney, liver, small intestine, placenta, lung, heart and skeletal muscle. Ref.1 Ref.4

Sequence similarities: Belongs to the short-chain dehydrogenases/reductases (SDR) family.