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Western Blot (WB) (RDH10 Antibody (Center) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).)

Rabbit RDH10 Polyclonal Antibody | anti-RDH10 antibody

RDH10 Antibody (Center)

Gene Names
RDH10; SDR16C4
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RDH10; Polyclonal Antibody; RDH10 Antibody (Center); Retinol dehydrogenase 10; anti-RDH10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RDH10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human RDH10.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
106-135
Sequence Length
341
Applicable Applications for anti-RDH10 antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(RDH10 Antibody (Center) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).)

Western Blot (WB) (RDH10 Antibody (Center) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the RDH10 antibody detected the RDH10 protein (arrow).)

Immunohistochemistry (IHC)

(RDH10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (RDH10 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RDH10 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(RDH10 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (RDH10 Antibody (Center) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-RDH10 antibody
RDH10 generates all-trans retinal from all-trans retinol and may plan an important role in the photic visual cycle. All-trans retinal is isomerized to 11-cis retinal by the retinal G protein-coupled receptor (RGR; MIM 600342) when the retinal pigment epithelium (RPE) is illuminated.
Product Categories/Family for anti-RDH10 antibody
References
Bankovic, J., et al. Lung Cancer 67(2):151-159(2010)
Takahashi, Y., et al. Biochem. J. 419(1):113-122(2009)
Persson, B., et al. Chem. Biol. Interact. 178 (1-3), 94-98 (2009)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38087
NCBI Official Full Name
retinol dehydrogenase 10
NCBI Official Synonym Full Names
retinol dehydrogenase 10 (all-trans)
NCBI Official Symbol
RDH10
NCBI Official Synonym Symbols
SDR16C4
NCBI Protein Information
retinol dehydrogenase 10
UniProt Protein Name
Retinol dehydrogenase 10
Protein Family
UniProt Gene Name
RDH10
UniProt Synonym Gene Names
SDR16C4
UniProt Entry Name
RDH10_HUMAN

NCBI Description

This gene encodes a retinol dehydrogenase, which converts all-trans-retinol to all-trans-retinal, with preference for NADP as a cofactor. Studies in mice suggest that this protein is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development. [provided by RefSeq, Dec 2011]

Uniprot Description

RDH10: Retinol dehydrogenase with a clear preference for NADP. Converts all-trans-retinol to all-trans-retinal. Has no detectable activity towards 11-cis-retinol, 9-cis-retinol and 13-cis-retinol. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; Membrane protein, integral; EC 1.1.1.300

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: endoplasmic reticulum membrane; cytoplasm; integral to membrane; nucleus

Molecular Function: retinol dehydrogenase activity

Biological Process: embryonic forelimb morphogenesis; phototransduction, visible light; gonad development; retinal metabolic process; neural crest cell development; in utero embryonic development; ear development; embryonic viscerocranium morphogenesis; embryonic camera-type eye development; visual perception; retinol metabolic process; retinoid metabolic process; nose development; metanephros development

Research Articles on RDH10

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Product Notes

The RDH10 rdh10 (Catalog #AAA9214120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 106-135. The RDH10 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's RDH10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the RDH10 rdh10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RDH10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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