Human Kruppel Like Factor 4, Gut (KLF4) ELISA Kit | KLF4 elisa kit

Human Kruppel Like Factor 4, Gut (KLF4) ELISA Kit

Cat. Num. AAA2703063

• Gene Names: KLF4; EZF; GKLF
• Reactivity: Human
• Synonyms: Kruppel Like Factor 4; Gut (KLF4); Human Kruppel Like Factor 4; Gut (KLF4) ELISA Kit; EZF; GKLF; Gut-Enriched Krüppel-Like Factor; Epithelial zinc finger protein EZF; KLF4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kruppel Like Factor 4, Gut (KLF4).; No significant cross-reactivity or interference between Kruppel Like Factor 4, Gut (KLF4) and analogues was observed.
• Sequence Length: 63

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLF4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 4, Gut (KLF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 4, Gut (KLF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 4, Gut (KLF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kruppel Like Factor 4, Gut (KLF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 194248077
• NCBI GeneID: 9314
• NCBI Accession #: NP_004226.3
• NCBI GenBank Nucleotide #: NM_004235.5
• UniProt Accession #: O43474; P78338; Q5T3J8; Q5T3J9; Q8N717; Q9UNP3; B2R8S4; B3KT79; L0R3I6; L0R4N5
• Molecular Weight: 6,489 Da
• NCBI Official Full Name: Krueppel-like factor 4 isoform 2
• NCBI Official Synonym Full Names: Kruppel like factor 4
• NCBI Official Symbol: KLF4
• NCBI Official Synonym Symbols: EZF; GKLF
• NCBI Protein Information: Krueppel-like factor 4
• UniProt Protein Name: Krueppel-like factor 4
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF4
• UniProt Synonym Gene Names: EZF; GKLF

NCBI Description

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.