Western Blot (WB)
(Western blot analysis of extracts from K562 cells using RCL antibody.)
Rabbit anti-Human RCL Polyclonal Antibody | anti-RCL antibody
RCL Antibody
Gene Names
DNPH1; RCL; C6orf108; dJ330M21.3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RCL; Polyclonal Antibody; RCL Antibody; 2' deoxynucleoside 5' phosphate N hydrolase 1; BC048355; c Myc responsive; c Myc responsive protein Rcl; c-Myc-responsive protein Rcl; C6orf108; C76683; Chromosome 6 open reading frame 108; Deoxyribonucleoside 5' monophosphate N glycosidase; Deoxyribonucleoside 5''-monophosphate N-glycosidase; dJ330M21.3; DNPH1; MGC54855; Putative c Myc responsive; Rcl; RCL_HUMAN; RP3 330M21.3; anti-RCL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
RCL antibody detects endogenous levels of total RCL
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
174
Applicable Applications for anti-RCL antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Cytoplasm.
Tissue Specificity
Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers.
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (80%), Bovine (90%), Sheep (90%), Rabbit (80%), Dog (90%), Xenopus (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from K562 cells using RCL antibody.)
Western Blot (WB)
(Western blot analysis of extracts from K562 cells using RCL antibody.)
Immunofluorescene (IF)
(MBS9605407 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9605407 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-RCL antibody
Function: Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.
Subunit Structure: Monomer and homodimer.
Similarity: Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.
Subunit Structure: Monomer and homodimer.
Similarity: Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 22 kD
Predicted: 20 kDa
Predicted: 20 kDa
NCBI Official Full Name
2'-deoxynucleoside 5'-phosphate N-hydrolase 1 isoform 1
NCBI Official Synonym Full Names
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
NCBI Official Symbol
DNPH1
NCBI Official Synonym Symbols
RCL; C6orf108; dJ330M21.3
NCBI Protein Information
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
UniProt Protein Name
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
UniProt Gene Name
DNPH1
UniProt Synonym Gene Names
C6orf108; RCLUniRule annotationManual assertion according to rulesiHAMAP-Rule:MF_03036
NCBI Description
This gene was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The exact function of this gene is not known but studies in rat suggest a role in cellular proliferation and c-Myc-mediated transformation. Two alternative transcripts encoding different proteins have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.
Research Articles on RCL
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Product Notes
The RCL dnph1 (Catalog #AAA9605407) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RCL Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RCL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the RCL dnph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RCL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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