Rabbit anti-Human RCL Polyclonal Antibody | anti-RCL antibody

RCL polyclonal antibody

Cat. Num. AAA3002282

• Gene Names: DNPH1; RCL; C6orf108; dJ330M21.3; RP3-330M21.3
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RCL; Polyclonal Antibody; RCL polyclonal antibody; C6orf108; Deoxyribonucleoside 5'-monophosphate N-glycosidase; c-Myc-responsive protein Rcl; anti-RCL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: RCL Polyclonal Antibody detects endogenous levels of RCL protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applicable Applications for anti-RCL antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1/500-1/2000; ELISA: 1/40,000
• Immunogen: The antiserum was produced against synthesized peptide derived from human RCL. AA range: 1-50
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using RCL Polyclonal Antibody)

Western Blot (WB)

(Western blot analysis of lysates from Jurkat and K562 cells, using RCL Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of the lysates from 293 cells using RCL antibody.)

Related Product Information for anti-RCL antibody

Background: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1(DNPH1) Homo sapiens. This gene was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The exact function of this gene is not known but studies in rat suggest a role in cellular proliferation and c-Myc-mediated transformation. Two alternative transcripts encoding different proteins have been described. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

• NCBI GI #: 5454002
• NCBI GeneID: 10591
• NCBI Accession #: NP_006434.1
• NCBI GenBank Nucleotide #: NM_006443.2
• UniProt Accession #: O43598; B2LUJ9
• Molecular Weight: 19,108 Da
• NCBI Official Full Name: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 isoform 1
• NCBI Official Synonym Full Names: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1
• NCBI Official Symbol: DNPH1
• NCBI Official Synonym Symbols: RCL; C6orf108; dJ330M21.3; RP3-330M21.3
• NCBI Protein Information: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1; putative c-Myc-responsive; c-Myc-responsive protein Rcl; deoxyribonucleoside 5'-monophosphate N-glycosidase
• UniProt Protein Name: 2'-deoxynucleoside 5'-phosphate N-hydrolase 1
• UniProt Gene Name: DNPH1
• UniProt Synonym Gene Names: C6orf108; RCL
• UniProt Entry Name: DNPH1_HUMAN

NCBI Description

This gene was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The exact function of this gene is not known but studies in rat suggest a role in cellular proliferation and c-Myc-mediated transformation. Two alternative transcripts encoding different proteins have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

DNPH1: Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5- phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'- monophosphates containing purine bases are preferred to those containing pyrimidine bases. Monomer and homodimer. Expression is induced by ETV1. Expressed at low levels in brain, colon, lung, peripheral blood leukocytes, placenta, small intestine, and thymus. Expressed at high levels in heart, kidney, liver, skeletal muscle and spleen. Overexpressed in a significant proportion of breast cancers. Belongs to the deoxyribonucleoside 5'-monophosphate N- glycosidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.-; Hydrolase

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein homodimerization activity; nucleoside deoxyribosyltransferase activity

Biological Process: cell proliferation; epithelial cell differentiation; nucleoside metabolic process; nucleotide metabolic process; positive regulation of cell growth; deoxyribonucleoside monophosphate catabolic process