SDS-Page
RCL recombinant protein
RCL, 1-174aa,Human, His tag, E Coli
Gene Names
DNPH1; RCL; C6orf108; dJ330M21.3
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
RCL; 1-174aa; Human; His tag; E Coli; Deoxyribonucleoside 5'-monophosphate N-glycosidase isoform 1; c-Myc-responsive protein Rcl; C6orf108; RCL recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MAAAMVPGRS ESWERGEPGR PALYFCGSIR GGREDRTLYE RIVSRLRRFG TVLTEHVAAA ELGARGEEAA GGDRLIHEQD LEWLQQADVV VAEVTQPSLG VGYELGRAVA FNKRILCLFR PQSGRVLSAM IRGAADGSRF QVWDYEEGEV EALLDRYFEA DPPGQVAASP DPTT
Sequence Length
174
Applicable Applications for RCL recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for RCL recombinant protein
Deoxyribonucleoside 5'-monophosphate N-glycosidase isoform 1, also known as RCL, belonged to the N-deoxyribosyltransferase family. This protein catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. RCL was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. Recombinant human RCL protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for RCL recombinant protein
References
Ghiorghi Y K., et al. (2007) J Biol Chem. 282 (11):8150-6.; Munqall A J., et al. (2003) Nature. 425 (6960):805-11.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21.2 kDa (194aa), confirmed by MALDI-TOF
NCBI Official Full Name
2'-deoxynucleoside 5'-phosphate N-hydrolase 1 isoform 1
NCBI Official Synonym Full Names
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
NCBI Official Symbol
DNPH1
NCBI Official Synonym Symbols
RCL; C6orf108; dJ330M21.3
NCBI Protein Information
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
UniProt Protein Name
2'-deoxynucleoside 5'-phosphate N-hydrolase 1
UniProt Gene Name
DNPH1
NCBI Description
This gene was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The exact function of this gene is not known but studies in rat suggest a role in cellular proliferation and c-Myc-mediated transformation. Two alternative transcripts encoding different proteins have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.
Research Articles on RCL
Similar Products
Product Notes
The RCL dnph1 (Catalog #AAA203618) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's RCL can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the RCL dnph1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MAAAMVPGRS ESWERGEPGR PALYFCGSIR GGREDRTLYE RIVSRLRRFG TVLTEHVAAA ELGARGEEAA GGDRLIHEQD LEWLQQADVV VAEVTQPSLG VGYELGRAVA FNKRILCLFR PQSGRVLSAM IRGAADGSRF QVWDYEEGEV EALLDRYFEA DPPGQVAASP DPTT. It is sometimes possible for the material contained within the vial of "RCL, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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