SDS-PAGE
Nucleoside phosphorylase DeoD-type Recombinant Protein | deoD recombinant protein
Recombinant E Coli Purine nucleoside phosphorylase DeoD-type
Gene Names
deoD; ECK4376; JW4347; pup
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Nucleoside phosphorylase DeoD-type; Recombinant E Coli Purine nucleoside phosphorylase DeoD-type; Inosine phosphorylase; deoD recombinant protein
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
2-239aa; Full Length
Sequence
ATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
Sequence Length
239
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for deoD recombinant protein
Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.
References
Use of site-directed mutagenesis to enhance the epitope-shielding effect of covalent modification of proteins with polyethylene glycol.Hershfield M.S., Chaffee S., Koro-Johnson L., Mary A., Smith A.A., Short S.A.Proc. Natl. Acad. Sci. U.S.A. 88:7185-7189(1991)
Analysis of the Escherichia coli genome VI
DNA sequence of the region from 92.8 through 100 minutes.Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.Nucleic Acids Res. 23:2105-2119(1995)
The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997)
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006)
Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.Henzel W.J., Billeci T.M., Stults J.T., Wong S.C., Grimley C., Watanabe C.Proc. Natl. Acad. Sci. U.S.A. 90:5011-5015(1993)
Analysis of the terminator region after the deoCABD operon of Escherichia coli K-12 using a new class of single copy number operon-fusion vectors.Larsen J.E.L., Albrechtsen B., Valentin-Hansen P.Nucleic Acids Res. 15:5125-5140(1987)
Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.Mol. Cell. Proteomics 8:215-225(2009)
The crystal structure of Escherichia coli purine nucleoside phosphorylase
a comparison with the human enzyme reveals a conserved topology.Mao C., Cook W.J., Zhou M., Koszalka G.W., Krenitsky T.A., Ealick S.E.Structure 5:1373-1383(1997)
Crystal structure of the ternary complex of E. coli purine nucleoside phosphorylase with formycin B, a structural analogue of the substrate inosine, and phosphate (sulphate)
at 2.1-A resolution.Koellner G., Luic M., Shugar D., Saenger W., Bzowska A.J. Mol. Biol. 280:153-166(1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52.8 kDa
NCBI Official Full Name
purine nucleoside phosphorylase 1; nicotinamide 1-beta-D-riboside phosphorylase
NCBI Official Symbol
deoD
NCBI Official Synonym Symbols
ECK4376; JW4347; pup
NCBI Protein Information
purine nucleoside phosphorylase 1; nicotinamide 1-beta-D-riboside phosphorylase
UniProt Protein Name
Purine nucleoside phosphorylase DeoD-type
Protein Family
UniProt Gene Name
deoD
UniProt Synonym Gene Names
pup; PNP
UniProt Entry Name
DEOD_ECOLI
NCBI Description
DeoD has reversible purine nucleoside phosphorylase (PNP) activity with broad substrate specificity; however, it does not act on xanthosine or pyrimidine nucleosides . [More information is available at EcoCyc: EG10222].
Uniprot Description
Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules.
Research Articles on deoD
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Product Notes
The deoD deod (Catalog #AAA1265176) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-239aa; Full Length. The amino acid sequence is listed below: ATPHINAEMG DFADVVLMPG DPLRAKYIAE TFLEDAREVN NVRGMLGFTG TYKGRKISVM GHGMGIPSCS IYTKELITDF GVKKIIRVGS CGAVLPHVKL RDVVIGMGAC TDSKVNRIRF KDHDFAAIAD FDMVRNAVDA AKALGIDARV GNLFSADLFY SPDGEMFDVM EKYGILGVEM EAAGIYGVAA EFGAKALTIC TVSDHIRTHE QTTAAERQTT FNDMIKIALE SVLLGDKE. It is sometimes possible for the material contained within the vial of "Nucleoside phosphorylase DeoD-type, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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